Liquid purification or separation – Processes – Treating by enzyme
Patent
1997-01-28
1997-12-30
Wyse, Thomas G.
Liquid purification or separation
Processes
Treating by enzyme
210764, 162161, 422 28, 435183, 4352521, 435264, 435267, 435822, C12N 924, C02F 150, D21F 166
Patent
active
057026050
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a slime-decomposing enzyme producing strain of microorganism which is capable of producing an enzyme useful for decomposing slime and a process for producing said slime-decomposing enzyme.
BACKGROUND TECHNOLOGY
A slime is a viscous mud-like substance composed predominantly of cellular components of microorganisms and products of their metabolism either suspended in a body of utility water or deposited on the wall surfaces of water lines and tanks. In particular, a slime is formed in paper-mill white water, gets deposited, grow, and as it attains a certain size, becomes exfoliated to cause various troubles in the paper-making industry.
It is known that not only bacteria but also Eumycetes inclusive of molds and yeasts exist in white water. Particularly in recent years, eutrophication of white water due to recycling of white water, an increased proportion of used paper in paper stock, and addition of starch, etc. has generated the problem of mold slime.
For control of formation of slimes, a variety of antimicrobial agents have heretofore been employed. Among them, organic nitrogen compounds, organosulfur compounds, organic nitrogen- and sulfur-containing compounds, and organobromine compounds are known.
With regard to microbicides and antimicrobial agents for slime control, the use of safety agents benign to the environment has been required. However, the appearance of resistant strains as well as pollution problems remain to be solved as yet.
As a class of biological preparations for slime control, various enzymatic agents are also known. For example, Japanese Kokoku Publication Sho-53-39395 (U.S. Pat. No. 3,773,623) discloses the use of levan hydrolase for decomposing levan, a product of metabolism in slime-forming microorganisms, for slime control. Being a kind of polysaccharide produced by bacteria, levan is a .beta.-fructan with a backbone chain composed of .beta.-2,6-fructofuranose residues.
Japanese Kokai Publication Sho-59-225103 (U.S. Pat. No. 4,684,469) discloses a binary composition comprising a biocidal substance and a polysaccharide-decomposing enzyme which includes levan hydrolase, dextrin hydrolase, and amylase.
Japanese Kokai Publication Hei-3-193 describes that a slime can be removed effectively by using .beta.-glucanase in combination with protease and amylase to disrupt the surface layer of a slime.
Japanese Kokai Publication Hei-4-91288 proposes the use of a .beta.-glycosidase.
In using such an enzymatic agents, appearance of resistant strains and hazards, which are the drawbacks of antimicrobial agents, need not be considered. However, the representative enzyme levan hydrolase and the above-mentioned binary biocidal substance, .beta.-glucanase and the like are useful for bacterial slimes but not effective enough for mold slimes, due to their specificity.
Other commercial .beta.-glucosidase are not effective enough when used alone and must be used in combination with other kinds of enzymes. Moreover, they are expensive, difficult to formulate, and therefore, not suited for exploitation on an industrial scale.
SUMMARY OF THE INVENTION
In view of the above state of the art and for the purpose of screening out microorganisms capable of producing enzymes effective for Eumycetes slimes originating in molds, yeasts and the like, the inventors of the present invention screened a variety of microorganisms from soil and the like and, as a consequence, discovered a novel slime-decomposing enzyme producing strain of microorganism capable of producing an enzyme which can decompose slimes originating in various molds, yeasts and the like with a broad specificity. The present invention has been developed on the basis of the above finding.
The essence of the present invention resides in the very slime-decomposing enzyme producing strain of microorganism which belongs to the genus Cellulomonas and is capable of producing an enzyme effective for decomposition of a slime. The present invention is further directed to a process for producing a slime-dec
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patent: 4169762 (1979-10-01), Hitzman
patent: 4941533 (1990-07-01), Buller et al.
patent: 5212079 (1993-05-01), Fujio et al.
patent: 5545801 (1996-08-01), Fulton
Sanyo Chemical Industries Ltd.
Wyse Thomas G.
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