Skin substitutes with improved barrier function

Details Skin substitutes with improved barrier function Skin substitutes with improved barrier function

2005-12-13

2005-12-13

Chen, Shin-Lin (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

C435S325000, C435S395000, C623S015110, C623S015120

Reexamination Certificate

active

06974697

ABSTRACT:
The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.

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