Skin substitutes with improved barrier function

Details Skin substitutes with improved barrier function Skin substitutes with improved barrier function

2011-06-07

2011-06-07

Chen, Shin-Lin (Department: 1632)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S325000, C435S371000, C435S395000, C623S015110, C623S015120

Reexamination Certificate

active

07955790

ABSTRACT:
The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.

REFERENCES:
patent: 4485096 (1984-11-01), Bell
patent: 4707354 (1987-11-01), Garlen et al.
patent: 5536656 (1996-07-01), Kemp et al.
patent: 5658331 (1997-08-01), Della Valle et al.
patent: 5693332 (1997-12-01), Hansbrough
patent: 5968546 (1999-10-01), Baur et al.
patent: 5989837 (1999-11-01), Allen-Hoffmann et al.
patent: 5994115 (1999-11-01), Meyers
patent: 6039760 (2000-03-01), Eisenberg
patent: 6074859 (2000-06-01), Hirokawa et al.
patent: 6214567 (2001-04-01), Allen-Hoffmann
patent: 6485724 (2002-11-01), Allen-Hoffmann
patent: 6495135 (2002-12-01), Allen-Hoffmann
patent: 6514711 (2003-02-01), Allen-Hoffmann
patent: 6846675 (2005-01-01), Conrad et al.
patent: 6884595 (2005-04-01), Allen-Hoffmann
patent: 6964869 (2005-11-01), Allen-Hoffmann
patent: 6974697 (2005-12-01), Comer et al.
patent: 7407805 (2008-08-01), Comer et al.
patent: 2001/0023061 (2001-09-01), Allen-Hoffmann
patent: 2002/0102726 (2002-08-01), Allen-Hoffmann
patent: 2002/0142282 (2002-10-01), Allen-Hoffmann
patent: 2002/0164793 (2002-11-01), Conrad
patent: 2002/0168768 (2002-11-01), Comer et al.
patent: 2002/0187498 (2002-12-01), Comer
patent: 2002/0192196 (2002-12-01), Allen-Hoffmann
patent: 2003/0157476 (2003-08-01), Allen-Hoffmann
patent: 2004/0146881 (2004-07-01), Allen-Hoffmann
patent: 2004/0265787 (2004-12-01), Allen-Hoffmann
patent: 2005/0079578 (2005-04-01), Centanni
patent: 2005/0186185 (2005-08-01), Conrad
patent: 2005/0226853 (2005-10-01), Conrad
patent: 2006/0030044 (2006-02-01), Allen-Hoffmann
patent: 2006/0121608 (2006-06-01), Comer
patent: 2006/0222635 (2006-10-01), Centanni
patent: 2006/0257383 (2006-11-01), Allen-Hoffmann
patent: 2006/0258001 (2006-11-01), Allen-Hoffmann
Augustin et al., 1997, Photodermatol Photoimmunol Photomed, vol. 13, No. 1-2, p. 27-36.
Sando et al., “Induction of Ceramide Glucosyltransferase Activity in Cultured Human Keratinocytes” J. Biol. Chem., (1996) 271 (36): 22044-51.
Watanabe et al., “Up-regulation of Glucosylceramide Synthase Expression and Activity during Human Keratinocyte Differentiation*,” J. Biol. Chem., (1998) 273(16): 9651-5.
Ponec et al., “The Formation of Competent Barrier Lipids in Reconstructed Human Epidermis Requires the Presence of Vitamin C” J. Invest. Dermatol., (1997) 109(3): 348-55.
Denda et al., “Exposure to a Dry Environment Enhances Epidermal Permeability Barrier Function, ”J. Invest. Dermatol., (1998) 111(5): 858-63.
Hanley et al., “Activators of the Nuclear Hormone Receptors PPARa and FXR Accelerate the Development of the Fetal Epidermal Permeability Barrier,” J. Clin. Invest., (1997) 00(3): p. 705-12.
Hanley et al., “Fetal Epidermal Differentiation and Barrier Development in Vivo is Accelerated by Nuclear Hormone Receptor Activators” J. Invest. Dermatol., (1999) 113(5): 788-95.
Zhang et al., “The Gut-enriched Kruppel-like Factor Suppresses the Activity of the CYP1A1 Promoter in an Sp1-dependent Fashion*,” J. Biol. Chem., (1998) 273(28): 17917-25.
Dewet et al, “Firefly Luciferase Gene: Structure and Expression in Mammalian Cells,” Mol. Cell. Biol. (1987) 7:725.
Smith and Waterman, “Comparison of Biosequences,” Adv. Appl. Math. (1981) 2: 482.
Needleman and Wunsch, A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins, J. Mol. Biol. (1970) 48:443.
Pearson and Lipman, “Improved tools for biological sequence comparison” Proc. Natl. Acad. Sci. (U.S.A.) (1988) 85:2444.
Graham and Van Der Eb, “A New Technique for the Assay of Infectivity of Human Adenovirus 5 DNA,” Virol., (1973) 52:456.
Fuchs, “Epidermal differentiation and keratin gene expression,” J. Cell. Sci. Supp., (1993) 17: 197-208.
Schurer et al., “Stratum Corneum Lipid Function,” Dermatologica, (1991)183: 77-94.
Aeschlimann et al., “Transglbtaminases: Protein Cross-linking Enzymes in Tissues and Body Fluids,” Thrombosis & Haemostasis, (1994) 71(4): 402-15.
Reichert et al., “The cornified envelope: a key structure of terminally differentiating keratinocytes,” M. Dannon, Editor. 1993, Academic Press, Inc.: San Diego. (1993)107-150.
Sachsenmeier et al., “Transforming Growth Factor-B1 Inhibits Nucleosomal Fragmentation in Human Keratinocytes following Loss of Adhesion*,” J. Bio1. Chem., (1996) 271: 5-8.
Hines et al., “Analysis of DNA Fragmentation in Epidermal Keratinocytes using the Apoptosis Detection System, Fluorescein” Promega Notes, (1996) 59: p. 30-36.
Anderson and Young, “Quantitative Filter Hybridisation,” Quantitative Hybridisation (1985).
Hines et al, “Keratinocyte Growth Factor Inhibits Cross-linked Envelops Formation and Nucleosomal Fragmentation in Cultured Human Keratinocytes*,” J. Biol. Chem., (1996) 271(11): 6245-6251.
Polakowska et al., “Apoptosis in Human Skin Development: Morphogenesis, Periderm, and Stern Cells,” Developmental Dynamics, (1994) 199(3): 176-88.
Haake et al., “Apoptosis: A Role in Skin Aging?” J. Invest. Derm, Symp. Proc., (1998) 3: 28-35.
Wertz et al., “The physical, chemical and functional properties of lipids in the skin and other biological barriers,” Chem. Phys. Lipids., (1998) 91(2): 85-96.
Meana et al., “Large surface of cultured human epithelium obtained on a dermal matrix based on live fibroblast-containing fibrin gels,” Bums (1998) 24:621-30.
Baden, “Isolation and Characterization of a Spontaneously Arising Long-Lived Line of Human Keratinocytes NM 1),” In Vitro Cell. Dev. Biol. (1987) 23(3):205-213.
Boucamp et al.,“Normal Keratinization in a Spontaneously Immortalized Aneuploid Human Keratinocyte Cell Line,” J. cell. Boil. (1988)106:761-771.
Bell et al., “Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro,” Prec. Nat. Acad. Sci. USA, (1979) 76: 1274-1278.
Fusenig, “Epithelial mesenchymal interactions regulate keratinocyte growth and differentiation in vitro,” in The Keratinocyte Handbook, (1994) pp. 71-94.
Parenteau et al., “The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function,” Cytotechnology, (1992) 9: 163-171.
Cumpstone et al., “The Water Permeability of Primary Mouse Keratinocyte Cultures Grown at the Air-Liquid Interface,” J. Invest. Dermatol., (1989) 92(4): 598-600.
Ponec, “In vitro cultured human skin cells as alternatives to animals for skin irritancy screening,” Int. J. Cosmetic Sci., (1992) 14: 245-264.
Mak et al. “Barrier Function of Human Keratinocyte Cultures Grown at the Air-Liquid Interface,” J. Invest. Dermatol., (1991) 96(3): 323-7 (1991).
Grubauer et al., “Transepidermal water loss: the signal for recovery of barrier structure and function,” J. Lipid Res., (1989) 30(3): 323-33.
Williams et al., “Ontogeny of the epidermal permeability barrier,” J Investig. Dermatol. Symp. Proc., (1998) 3(2): 75-9.
Vicanova et al. “Stratum corneum lipid composition and structure in cultured skin substitutes is restored to normal after grafting onto athymic mice,” J. Investig. Dermatol. Symp. Proc.,(1998) 3(2): 114-20.
Aszterbaum et al, “Ontogeny of

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