SKAP55 compositions and methods of use therefor

Details SKAP55 compositions and methods of use therefor SKAP55 compositions and methods of use therefor

1996-09-13

1999-08-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435325, 4352523, 43525233, 4353201, 536 231, 536 234, 536 235, 530350, C12P 2106, C07H 2104, C12N 120

Patent

active

059424150

ABSTRACT:
Isolated nucleic acid molecules encoding a novel protein, SKAP55, that interacts with the protein tyrosine kinase Fyn, are disclosed. SKAP55 protein has an apparent native molecular weight of 55 kDa, an isoelectric point of 4.3 and contains a pleckstrin homology (PH) domain and a src homology 3 (SH3) domain. In addition to isolated nucleic acids molecules encoding SKAP55 protein, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a SKAP55 transgene. The invention further provides isolated SKAP55 proteins and peptides, SKAP55 fusion proteins and anti-SKAP55 antibodies. Methods of using the SKAP55 compositions of the invention are also disclosed, including methods for detecting SKAP55 protein or mRNA in a biological sample, methods of modulating SKAP55 activity in a cell, and methods for identifying agents that modulate an interaction between SKAP55 and Fyn.

REFERENCES:
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