Size markers for electrophoretic analysis of DNA

Details Size markers for electrophoretic analysis of DNA Size markers for electrophoretic analysis of DNA

1990-07-13

1994-05-31

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435967, 436 8, C12Q 168

Patent

active

053169085

ABSTRACT:
The present invention discloses a DNA marker ladder useful in Southern blot hybridizations. The ladder is made up of pooled DNA restriction endonuclease digests, where each restriction digest contains at least one fragment complementary to a probe and at least one fragment not complementary to the probe. The regions of complementarity between the probe and the complementary fragments are double-stranded segments of the fragments. The ladder is characterized by an approximately even spacing of bands, resulting from choosing fragments having an logarithmic size distribution. Kits incorporating this ladder and a probe or means for making a probe or a probe and a means for labeling a probe are also disclosed.

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