Six base oligonucleotide linkers and methods for their use

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4351723, 435 91, 536 27, 935 10, C12N 1500, C12P 1934, C07H 1512

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047191791

ABSTRACT:
A method for inserting a selected restriction site into a double stranded genetic sequence at a selected tab site, and a linker for use therewith are disclosed. The method involves treating the genetic sequence with an opening agent (such as a restriction enzyme) so as to open both strands of the tab site. One then exposes the opened genetic sequence to two hexameric single stranded oligonucleotide linkers in the presence of a ligating enzyme. The two linkers have the same nitrogenous base sequence, a sequence which is partially palindromic complementary to itself at least one end of the nitrogenous sequence, but is not completely palindromic complementary to itself. Through use of this method, the two linkers are inserted in the opening with partial complementary overlap, and at least one of the linkers is affixed to each strand. Subsequently, the site is reclosed at the point of the insertion so as to contain a six base insertion.

REFERENCES:
G. R. Gough et al., 23 Tetrahedron Letters, 3439-3442 (1982).
J. Stawinski et al., 4 Nucleic Acids Research, 353-371 (1977).
R. Rothstein et al., 68 Meth. Enzym., 98-109 (1979).
R. Lathe et al., 20 Gene, 187-195 (1982).
J. Boeke, 181 Mol. Gen. Genet, 288-291 (1981).
J. Stone et al., 37 Cell, 549-558 (1984).
J. Vieira et al., 19 Gene, 259-268 (1982).
F. Heffron et al., 75 Proc. Natl. Acad. Sci., USA, 6012-6016 (1978).
C. Bahl et al., 1 Gene, 81-92 (1976).
C. Bahl et al., 81 Biochem. & Biophys., 685-703 (1978).
A. Maxam et al., 65 Meth. Enzym., 499-560 (1977).
A. Banaszuk et al., 128 Anal. Biochem., 281-286 (1983).
D. Hanahan, 166 J. Mol. Biol., 577-580 (1983).
D. Holmes et al., 114 Anal. Biochem., 193-197 (1981).
G. Gough et al., 7 Nucleic Acids Research, 1955-1964 (1979).
A. Sood et al., 4 Nucleic Acids Research, 2757-2765 (1977).
C. Reese et al., 30 Tetrahedron Letters, 2727-2730 (1978).
J. Stawinski et al., 54 Can. J. Chem., 670 (1976).
Barany, F., Gene, 37:111-123, 1985.
Barany, F., P.N.A.S.(USA), 82:4202-4206, 1985 (Jun.).
Roberts, R., Nucl. Ac. Research, 13:r165-r207, 1985.
Barton et al, P.N.A.S.(USA), 82:6460-6464, 1985 (Oct.).
Hertzberg et al., J. Am. Chem. Soc., 104:313-315, 1982.

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