Site-targeted transformation using amplification vectors

Details Site-targeted transformation using amplification vectors Site-targeted transformation using amplification vectors

2002-03-22

2011-11-15

Page, Brent T (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S280000, C800S288000, C800S295000, C435S320100, C435S440000, C435S468000, C435S469000, C536S023100, C536S023200, C536S023700, C536S024100

Reexamination Certificate

active

08058506

ABSTRACT:
A process of causing a targeted integration of DNA of interest into a plant cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous recombination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

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