Site-specific synthesis of pseudouridine in RNA

Details Site-specific synthesis of pseudouridine in RNA Site-specific synthesis of pseudouridine in RNA

1998-05-08

1999-11-23

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

435 6, 435 911, 4352542, 4353201, 536 231, 536 241, 536 2432, 536 245, C12Q 168, C12N 1581, C12N 1511, C07H 2104

Patent

active

059899115

ABSTRACT:
The invention features a modified small nucleolar ribonucleic acid (snoRNA) that directs the conversion of a uridine to a pseudouridine in a target nucleic acid, e.g., RNA, that includes first and second flanking regions located on either side of the uridine. The modified snoRNA includes a ribonucleotide sequence of a box H/ACA snoRNA including a Domain A sequence and a Domain B sequence, or a Domain A sequence and a Domain C sequence, wherein the snoRNA is modified in that the Domain A sequence is replaced by a first recognition sequence complementary to at least three consecutive nucleotides in the first flanking region in the target nucleic acid, and the Domain B or C sequence is replaced by a second recognition sequence complementary to at least three consecutive nucleotides in the second flanking region in the target nucleic acid.

REFERENCES:
Balakin, et al., "The RNA World of the Nucleolus: Two Major Families of Small RNAs Defined by Different Box Elements with Related Functions," Cell, vol. 86:823-834, (1996).
Caffarelli, et al., "Processing of the intron-encoded U16 and U18 snoRNAs: The conserved C and D boxes control both the processing reaction and the . . . ," The EMBO Journal, vol. 15:1121-1131 (1996).
Tsui, et al., "Absence of hisT-Mediated tRNA Pseudouridylation Results in a Uracil Requirement That Interferes with Escherichia coli K-12 Cell Division," Journal of Bacteriology, vol. 173:7395-7400 (1991).
Ganot, et al., "The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence . . . ," Genes & Development, vol. 11:941-956 (1997).
Hagervall, et al., "Role of tRNA modification in translational fidelity," Biochimica et Biophysica Acta, vol. 1050:263-266 (1990).
Hartshorne, et al., "A common core structure for U3 small nucleolar RNAs," Nucleic Acids Research, vol. 22:3354-3364 (1994).
Huang, et al., "Accumulation of U14 Small Nuclear RNA in Saccharomyces cerevisiae Requires Box C, Box D, and a 5',3' Terminal Stem," Molecular and Cellular Biology, vol. 12:4456-4463 (1992).
Jarmolowski, et al., "Identification of essential elements in U14 RNA of Saccharomyces cerevisiae," The EMBO Journal, vol. 9:4503-4509 (1990).
Kiss-Laszlo, et al., "Site-Specific Sugar Methylation of rRNA: A Novel Function for Small Nuclear RNAs," RNA 1996, The First Annual Meeting of the RNA Society, Abstract (1996).
Kiss-Laszlo, et al., "Site-Specific Ribose Methylation of Preribosomal RNA: A Novel Function for Small Nucleolar RNAs," Cell, vol. 85:1077-1088 (1996).
Tyc, et al., "U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus," The EMBO Journal, vol. 8:3113-3119 (1989).
Watkins, et al., "Elements essential for processing intronic U14 snoRNA are located at the termini of the mature snoRNA sequence and include conserved nucleotide boxes C and D," RNA, vol. 2:118-133 (1996).
Xia, et al., "Identification of specific nucleotide sequences and structural elements required for intronic U14 snoRNA processing," RNA, vol. 3:17-26 (1997).
Maden et al, Database Biosis on STN, "Eukaryotic ribosomal RNA: The recent excitement in the nucleotide modification problem," Abstract No. 99655451, Chromosoma, vol. 105:391-400, (1997).
Ni et al, "Small Nucleolar RNAs Direct Site-Specific Synthesis of Pseudouridine in Ribosomal RNA," Cell, vol. 89:565-573 (1997).
Peculis, "RNA Processing: Pocket Guides to Ribosomal RNA," Current Biology, vol. 7:R480-R482 (1997).
Tollervey et al, "Function and Synthesis of Small Nucleolar RNAs," Current Opinion in Cellular Biology, vol. 9:337-342 (1997).
International Search Report dated Jul. 6, 1998.

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