Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Reexamination Certificate
2006-11-14
2006-11-14
Wilson, Michael C. (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
C800S018000, C800S021000
Reexamination Certificate
active
07135608
ABSTRACT:
Site-specific recombinases provide a means of efficiently manipulating chromosomal sequences in mammalian cells in culture and in animals. Embryonic stem cells containing recombinase nucleic acid constructs that were expressed in the male germline simplify current protocols for producing mice bearing homologously recombined alleles that have been secondarily rearranged by a site-specific recombinase. In different ProCre strains, between 80% and 100% of the progeny that inherited a Cre target nucleic acid construct from males that were also heterozygous for a ProCre nucleic acid construct inherited the Cre-recombined target. ProCre nucleic acid constructs and recombined targets segregated in the first generation. When ES cells prepared from one ProCre line were transfected with vectors containing a loxP-flanked neomycin cassette, G418 resistant, homologously recombined clones, in which the loxP sites remained functional, were readily isolated.
REFERENCES:
patent: 4870009 (1989-09-01), Evans et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 5654182 (1997-08-01), Wahl et al.
patent: WO 92/15694 (1992-09-01), None
Gu et al. Science, vol. 265, pp. 103-106, 1994.
Zambrowicz et al. Biology of Reproduction, vol. 50, pp. 65-72, 1994.
Lakso et al., Proc. Natl. Acad. Sci, USA, vol. 93, pp. 5860-5865, Jun. 1996.
Onouchi et al. Mol. Gen. Genet., Bol. 247, pp. 653-660, 1995.
Wall et al. J. Dairy Science, vol. 80, pp. 2213-2224, Sep. 1997.
Mullins et al. J. Clin. Invest., vol. 98, pp. S37-S40, Sep. 1996.
Lewandoski et al. Current Biology, vol. 7, pp. 148-151, 1997.
Robl and Heideman, “Production of transgenic rats and rabbits,” Transgneic animal technology, pp. 265-270, 1994.
Ahearn et al., “Cloning and Sequence Analysis of the Mouse Genomic Locus Encoding the Largest Subunit of RNA PolymeraseII*”The Journal of Biological Chemistry262 (22):10695-10705 (1987).
Albanesi et al., “A cell- and developmental stage-specific promoter drives the expression of a truncatedc-kitprotein during mouse spermatid elongation”Development122:1291-1302 (1996).
Araki et al., “Site-specific Recombinase, R, Encoded by Yeast Plasmid pSR1”J. Mol. Biol.225:25-37 (1992).
Araki et al., “Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase”Proc. Natl. Acad. Sci. USA92:160-164 (1995).
Askew et al., “Site-Directed Point Mutations in Embryonic Stem Cells: a Gene-Targeting Tag-and-Exchange Strategy”Molecular and Cellular Biology13 (7):4115-4124 (1993).
Beddington et al., “Anin situtransgenic enzyme marker for the midgestation mouse embryo and the visualization of inner cell mass clones during early organogenesis”Development106:37-46 (1989).
Behringer et al., “Heart and bone tumors in transgenic mice”Proc. Natl. Acad. Sci. USA85:2648-2652 (1988).
Braun et al., “Genetically haploid spermatids are phenotypically diploid”Nature337:373-376 (1989).
Broach and Hicks, “Replication and Recombination Functions Associated with the Yeast Plasmid, 2μ Circle”Cell21:501-508 (1980).
Brusa et al., “Early-Onset Epilepsy and Postnatal Lethality Associated with an Editing-Deficient GluR-B Allele in Mice”Science270:1677-1680 (1995).
Caldwell and Handel, “Protamine transcript sharing among postmeiotic spermatids”Proc. Natl. Acad. Sci USA88:2407-2411 (1991).
Dzelzkalns et al., “Distinct cis-Acting Elements Direct Pistil-Specific and Pollen-Specific Activity of the Brassica S Locus Glycoprotein Gene Promotor”The Plant Cell5:855-863 (1993).
Evan et al., “Isolation of Monoclonal Antibodies Specific for Human c-myc Proto-Oncogene Product”Molecular and Cellular Biology5 (12):3610-3616 (1985).
Eyal et al., “Pollen Specificity Elements Reside in 30 bp of the Proximal Promotors of Two Pollen-Expressed Genes”The Plant Cell7:373-384 (1995).
Feil et al., “Ligand-activated site-specific recombination in mice”Proc. Natl. Acad. Sci USA93:10887-10890 (1996).
Fiering et al., “An in-out” strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: Analysis of the β-globin locus control regionProc. Natl. Acad. Sci. USA90:8469-8473 (1993).
Fiering et al., “Targeted deletion of 5'HS2 of the murine β-globin LCR reveals that it is not essential for proper regulation of the β-globin locus”Genes&Development9:2203-2213 (1995).
Gu et al., “Deletion of a DNA Polymerase β Gene Segment in T Cells Using Cell Type-Specific Gene Targeting”Science265:103-106 (1994).
Gu et al., “Independent Control of Immunoglobulin Switch Recombination at Individual Switch Regions Evidenced through Cre-loxP-Mediated Gene Targeting”Cell73:1155-1164 (1993).
Hasty et al., “Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells”Nature350:243-246 (1991).
Hecht et al., “Evidence fo Haploid Expression of Mouse Testicular Genes”Experimental Cell Research164:183-190 (1986).
Hoess et al., “P1 site-specific recombination: Nucleotide sequence of the recombining sites”Proc. Natl. Acad. Sci. USA79:3398-3401 (1982).
Howard et al., “Sperm-Specific Expression of Angiotensin-Converting Enzyme (ACE) Is Mediated by a 91-Base-Pair Promoter Containing a CRE-Like Element”Molecular and Cellular Biology13 (1):18-27 (1993).
Howard et al., “The VirD2 Protein of A. tumefaciens Contains a C-Terminal Bipartite Nuclear Localization Signal: Implications for Nuclear Uptake of DNA in Plant Cells”Cell68:109-118 (1992).
Jefferson et al., “GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants”The EMBO Journal6 (13):3901-3907 (1987).
Kalderon et al., “A Short Amino Acid Sequence Able to Specify Nuclear Location”Cell39:499-509 (1984).
King et al., “Deletion Mapping by Immunoselection against the H-Y Histocompatibility Antigen Further Resolves the SxraRegion of the Mouse Y Chromosome and Reveals Complexity of the Hya Locus” Genomics 24:159-168 (1994).
Kitamoto et al., “Humanized Prion Protein Knock-in by Cre-Induced Site-Specific Recombination in the Mouse”Biochemical and Biophysical Research Communications222 (3):742-747 (1996).
Klein et al., “High-velocity microprojectiles for delivering nucleic acids into living cells”Nature327:70-73 (1987).
Kozak, Marilyn, “Point Mutations Define a Sequence Flanking the AUG Initiator Codon That Modulates Translation by Eukaryotic Ribosomes”Cell44:283-292 (1986).
Kühn et al., “Inducible Gene Targeting in Mice”Science269:1427-1429 (1995).
Lakso et al., “Targeted oncogene activation by site-specific recombination in transgenic mice”Proc. Natl. Acad. Sci. USA89:6232-6236 (1992).
Lewandoski et al., “Zp3-cre, a transgenic mouse line for the activation or inactivation of loxP-flanked target genes specifically in the female germ line”Curr Biol7:148-151 (1997).
Logie et al., “Ligand-regulated site-specific recombination”Proc Natl Acad Sci92:5940-5944 (1995).
Mansour et al., “Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes”Nature336:348-352 (1988).
Nakamura and inouye, “DNA Sequence of the Gene for the Outer Membrane Lipoprotein ofE. coli: an Extremely AT-Rich Promoter”Cell18:1109-1117 (1979).
No et al., “Ecdysone-inducible gene expression in mammalian cells and transgenic mice”Proc. Natl. Acad. Sci USA93:3346-3351 (1996).
O'Gorman et al., “Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells”Reports251:1351-1355 (1991).
Olson et al., “Know Your Neighbors: three Phenotypes in Null Mutants of the Myogenic bHLH Gene M
O'Gorman Stephen
Wahl Geoffrey
Reiter Stephen E.
The Salk Institute for Biological Studies
Wilson Michael C.
LandOfFree
Site-specific recombination in eukaryotes and constructs... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Site-specific recombination in eukaryotes and constructs..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Site-specific recombination in eukaryotes and constructs... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3639548