Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-01-04
2005-01-04
Leffers, Gerry (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S455000, C435S462000, C435S091400, C435S091410, C435S091420, C424S093200, C424S093210, C514S04400A
Reexamination Certificate
active
06838285
ABSTRACT:
Site-specific recombinase based methods for making a recombinant adenoviral genome, as well as kits for practicing the same and the recombinant adenovirus vectors produced thereby, are provided. In the subject methods, the subject genomes are prepared from donor and acceptor vectors that each include at least one site recombinase recognition site, where in certain preferred embodiments, one of the donor and acceptor vectors includes a single recombinase recognition site while the other includes two recombinase recognition sites. The acceptor vector includes an adenoviral genome having an E region deletion. The donor vector includes an insertion nucleic acid. In the subject methods, the donor and acceptor vectors are combined in the presence of a recombinase to produce an adenoviral genome that includes the insertion nucleic acid. The subject adenoviral genomes find use in a variety of applications, including as vectors for use in a variety of applications.
REFERENCES:
patent: 5354678 (1994-10-01), Lebkowski et al.
patent: 5514253 (1996-05-01), Davis et al.
patent: 5585362 (1996-12-01), Wilson et al.
patent: 5589377 (1996-12-01), Lebkowski et al.
patent: 5616326 (1997-04-01), Spibey
patent: 5670488 (1997-09-01), Gregory et al.
patent: 5700470 (1997-12-01), Saito et al.
patent: 5731172 (1998-03-01), Saito et al.
patent: 5747072 (1998-05-01), Davidson et al.
patent: 5756283 (1998-05-01), Wilson et al.
patent: 5789390 (1998-08-01), Descamps et al.
patent: 5820868 (1998-10-01), Mittal et al.
patent: 5837484 (1998-11-01), Trempe et al.
patent: 5843742 (1998-12-01), Natsoulis et al.
patent: 5851806 (1998-12-01), Kovesdi et al.
patent: 5851808 (1998-12-01), Elledge et al.
patent: 5858351 (1999-01-01), Podsakoff et al.
patent: 5869037 (1999-02-01), Crystal et al.
patent: 5871982 (1999-02-01), Wilson et al.
patent: 5877011 (1999-03-01), Armentano et al.
patent: 5880102 (1999-03-01), George et al.
patent: 5885808 (1999-03-01), Spooner et al.
patent: 5888732 (1999-03-01), Hartley et al.
patent: 5891690 (1999-04-01), Massie
patent: 5919676 (1999-07-01), Graham et al.
patent: 5922576 (1999-07-01), He et al.
patent: 5928944 (1999-07-01), Seth et al.
patent: 5932210 (1999-08-01), Gregory et al.
patent: 5952221 (1999-09-01), Kurtzman et al.
patent: 5962311 (1999-10-01), Wickham et al.
patent: 5962313 (1999-10-01), Podsakoff et al.
patent: 5972700 (1999-10-01), Jacobs, Jr. et al.
patent: 6080569 (2000-06-01), Graham et al.
patent: 6120764 (2000-09-01), Graham et al.
patent: 6228646 (2001-05-01), Hardy
patent: 6274354 (2001-08-01), Wilson et al.
patent: 6287805 (2001-09-01), Graham et al.
patent: 6379943 (2002-04-01), Graham et al.
patent: 6403767 (2002-06-01), Graham et al.
patent: 6465254 (2002-10-01), Saito et al.
patent: 20020146392 (2002-10-01), Graham et al.
patent: WO 0012687 (2000-03-01), None
patent: WO 0030687 (2000-06-01), None
patent: WO0105961 (2001-01-01), None
Kijima et al. Applicaitn of the Cre Recombinase/loxP System Further Enhances Antitumor Effects in Cell type-specific Gene Therapy against Carcinoembyronic Antigen-producing Cancer. Cancer Research 59, 4906-4911, Oct. 1, 1999.*
Aoki K. et al. Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination in Vitro. Molecular Medicine. 5: pp224-231, 1999.*
Andre Lieber et al. “Recombinant Adenoviruses with Large Deletions Generated by Cre-Mediated Excision Exhibit Different Biological Properties Compared with First-generation Vectors In Vitro and In Vivo”Journal of Virology, Dec. 1996. p. 8944-8960, vol. 70 No. 12.
Farmer Andrew Alan
Quinn Thomas Patrick
Becton Dickinson
Leffers Gerry
Marvich Maria
Ogden Stasia L.
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