Site-specific preparation of polyethylene glycol-GRF conjugates

Details Site-specific preparation of polyethylene glycol-GRF conjugates Site-specific preparation of polyethylene glycol-GRF conjugates

2008-01-08

2008-01-08

Kam, Chih-Min (Department: 1656)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S002600, C530S399000, C530S345000, C530S344000, C530S333000, C530S324000, C530S406000, C424S198100, C424S009100

Reexamination Certificate

active

07317002

ABSTRACT:
A method is described for the site-specific preparation of hGRF-PEG conjugates containing one or more PEG units (per mole of hGRF) covalently bound to Lys12and/or Lys21and/or Nα, characterized in that the conjugation reaction between the hGRF peptide and activated PEG is carried out in solution and the desired hGRF-PEG conjugate can be purified by chromatography. The conjugates prepared by this method, as well as their use in the treatment, prevention of diagnosis of growth hormone deficiency, are also an object of the present invention.

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