Site-specific preparation of polyethlene glycol-GRF conjugates

Details Site-specific preparation of polyethlene glycol-GRF conjugates Site-specific preparation of polyethlene glycol-GRF conjugates

2005-03-22

2005-03-22

Weber, Jon (Department: 1653)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C530S399000, C530S345000, C530S344000, C530S333000, C530S324000, C530S406000, C424S198100, C435S069100

Reexamination Certificate

active

06869932

ABSTRACT:
A method is described for the site-specific preparation of hGRF-PEG conjugates containing one or more PEG units (per mole of hGRF) covalently bound to Lys12and/or Lys21and/or Nα, characterized in that the conjugation reaction between the hGRF peptide and activated PEG is carried out in solution and the desired hGRF-PEG conjugate can be purified by chromatography. The conjugates prepared by this method, as well as their use in the treatment, prevention or diagnosis of growth hormone deficiency, are also an object of the present invention.

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