Site specific binding system, imaging compositions and methods

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Magnetic imaging agent

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Details

424 9321, 424455, 4241781, 514937, A61B 5055, A61K 966, A61K 39395, A01N 4350

Patent

active

059895209

ABSTRACT:
A method for ligand-based binding of lipid encapsulated particles to molecular epitopes on a surface in vivo or in vitro comprises sequentially administering (a) a site-specific ligand activated with a biotin activating agent; (b) an avidin activating agent; and (c) lipid encapsulated particles activated with a biotin activating agent, whereby the ligand is conjugated to the particles through an avidin-biotin interaction and the resulting conjugate is bound to the molecular epitopes on such surface. The conjugate is effective for imaging by x-ray, ultrasound, magnetic resonance or positron emission tomography. Compositions for use in ultrasonic imaging of natural or synthetic surfaces and for enhancing the acoustic reflectivity thereof are also disclosed.

REFERENCES:
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patent: 5585112 (1996-12-01), Unger et al.
Urdal et al. "Tumor-associated Ganglio-N-Triosylceramide," Journal of Biological Chemistry, vol. 255, No. 21, pp. 10509-10516, 1980.

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