Site selective genomic modification of yeast of the genus pichia

Details Site selective genomic modification of yeast of the genus pichia Site selective genomic modification of yeast of the genus pichia

1985-10-25

1989-11-21

Teskin, Robin

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435320, 435 68, 435 70, 435 91, 435255, 435232, 435184, 435272, 536 27, 935 19, 935 28, 935 37, 935 55, 935 56, 935 69, C12N 0500, C12N 1500, C12P 1934

Patent

active

048822795

ABSTRACT:
Method for the site specific genomic modification of yeasts of the genus Pichia and novel DNA sequences useful therefor are provided. Pichia is transformed with a serially arranged linear DNA fragment comprising first and second insertable DNA fragments, which flank a marker gene. The insertable DNA sequences are homologous to defined portions of the Pichia genome and integrate by recombination at such defined sites. The resulting transformed organism contains the marker gene and any additional DNA sequences which are positioned between the insertable DNA fragments. In addition, the resulting transformed organism has either a disrupted or deleted sequence at the site of the genomic modification. Enhanced production of heterologous gene products is observed when using as the host for expression strains in which the alcohol oxidase gene has been disrupted. Upon disruption of the primary alcohol oxidase gene of Pichia, the existence of a second alcohol oxidase gene in Pichia was also discovered.

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