Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-05-07
1997-12-09
Elliott, George C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
536 231, 536 245, C12N 1509, C07H 2104
Patent
active
056959770
ABSTRACT:
Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.
REFERENCES:
patent: 5292662 (1994-03-01), Sandmeyer
Ann. Rev. Biochem. 1986. 55:631-61, Copyright 1986; Alan M. Weiner, Prescott L. Deiniger, Argiris Efstratiadis, "Nonviral Retroposons: Genes, Pseudogenes, and Transposable Elements Generated by the Reverse Flow of Genetic Information".
Proc. Natl. Acad. Sci. USA, vol.91, pp. 6064-6068, Jun. 1994, Biochemistry; Philippe Rouet, Fatima Smih, Maria Jasin, "Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells".
The EMBO Journal, vol.2, No. 5 pp.757-761, 1983; Marion Moos, Dieter Gallwitz, "Structure of two human .beta.-actin-related processed genes one of which is located next to a simple repetitive sequence".
Proc. Natl. Acad. Sci. USA, vol.92, pp.806-810, Jan. 1995, Biochemistry; David J. Segal, Dana Carroll, "Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes".
Nucleic Acids Research, vol. 13, No. 24, 1985, Gary R. Daniels, Prescott L. Deininger, "Integration site preferences of the Alu family and similar repetitive DNA sequences".
Cell, vol. 26, 11-17, Oct. 1981 (Part 1), Copyright 1981; Scott W. Van Arsdell, Richard A. Denison, Laurel B. Bernstein, Alan M. Weiner, "Direct Repeats Flank Three Small Nuclear RNA Pseudogenes in the Human Genome".
The EMBO Journal, vol. 14, No. 24, pp.6333-6338, 1985; Joel Maestre, Thierry Tchenio, Olivier Dhellin, Thierry Heidmann, "mRNA retroposition in human cells: processed pseudogene formation".
Elliott George C.
Genetic Information Research Institute
Schwartzman Robert
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