Site-directed mutagenesis of esterases

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4352625, 435440, C12N 918, C12N 1509, C12N 1555, A62D 300

Patent

active

060016256

ABSTRACT:
A method of modifying esterases by substitution with histadine of at least one amino acid within 6 .ANG. of an active site serine provides esterases useful for detoxifying organophasphates.

REFERENCES:
patent: 5312743 (1994-05-01), Schumacher et al.
G. Gibney et al., "Mutagenesis of Essential Functional Residues in Acetyllinesterase", Proc. Natl. Acad. Sci. 87: 7546-7550, Oct. 1990.

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