Chemistry: analytical and immunological testing – Involving production or treatment of antibody
Patent
1985-05-08
1988-10-04
Warden, Robert J.
Chemistry: analytical and immunological testing
Involving production or treatment of antibody
424 11, 424 858, 436548, 436804, 436808, 530389, 530402, 935107, G01N 33534, G01N 33537, A61K 4902
Patent
active
047756381
ABSTRACT:
A method for radiolabeling protein such as antibody which is performed in a single reaction vessel is described. A sealed reaction vessel having a port for addition and withdrawal of reagents preferably by syringe is used. Reagents for coupling radioisotope to the protein are added to the vessel. For radioiodination procedures, vessels can be pre-coated with the iodine coupling agent iodgen. The protein and the radioisotope are then added to the vessel and the radiolabel reaction allowed to proceed. After the reaction is complete, a resin is added to the vessel to adsorb the uncoupled radioisotope. The entire reaction mixture is then withdrawn from the vessel and the resin is separated from the protein preferably by sterile filtration.
REFERENCES:
patent: 4298590 (1981-11-01), Bogoch
patent: 4318902 (1982-03-01), Stephan
patent: 4348376 (1982-09-01), Goldenberg
N. R. Moudgal et al., "Pituitary Gonadotropins", in B. M. Jaffe et al., (Eds.), Methods of Hormone Radioimmunoassay, 2nd Ed., Academic Press, New York, 1979, pp. 173-198.
B. A. Roos et al., "Parathyroid Hormone", in B. M. Jaffe et al., (Eds.), IBID, pp. 401-418.
J. L. Vaitukaitis, "Specific Human Chorionic Gonadotropin Assay", in B. M. Jaffe et al., (Eds.), IBID, pp. 817-829.
Nature Directory of Biologicals: 1983 Buyers Guide, pp. 215 and 218.
Ferens, J. M. et al., J. Nucl. Med., 25:367:370, (1984).
Fraker, P. J. and Speck J. C., Biochem. Biophys. Res. Commun. 80:, 849-857, (1970).
Hudson, L. and Hay F. C., Practical Immunology, Blackwell Scientific Publications, pp. 239-244.
Method in Immunology and Immunochemistry, Williams, C. A. and Chase, M. W., Eds., Academic Press, New York, 1967, pp. 387-391.
Richardson, A. P., et al., Nucl. Med. Comm. 7:355-362, 1986.
Centocor, Inc.
Saunders David A.
Warden Robert J.
LandOfFree
Single vial technique for radiolabeling protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Single vial technique for radiolabeling protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single vial technique for radiolabeling protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2156251