Single-stranded labelled oligonucleotides of preselected sequenc

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 243, 536 2431, 536 2432, 435 6, C07H 2100

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058177868

ABSTRACT:
Substantially pure single-stranded oligonucleotides having a preselected sequence of not more than about 200 nucleotides, at least one of which is at a preselected position in the sequence and includes a base with a covalently attached linker arm containing or capable of binding at least one reporter group or solid support. A process for the chemical synthesis of the substantially pure single-stranded oligonucleotide and modified nucleosides useful in such synthesis are provided.

REFERENCES:
patent: 4247544 (1981-01-01), Bergstrom et al.
patent: 4267171 (1981-05-01), Bergstrom et al.
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4678667 (1987-07-01), Meares et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4794073 (1988-12-01), Dattagupta et al.
patent: 4795700 (1989-01-01), Dervan et al.
patent: 4808520 (1989-02-01), Dattagupta et al.
patent: 4818681 (1989-04-01), Dattagupta
patent: 4833251 (1989-05-01), Musso et al.
patent: 4849513 (1989-07-01), Smith et al.
patent: 4853327 (1989-08-01), Dattagupta
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 4910300 (1990-03-01), Urdea et al.
patent: 4948882 (1990-08-01), Ruth
patent: 5541313 (1996-07-01), Ruth
D. E. Draper, "Attachment of Reporter Groups to Specific, Selected Cytidine Residues in RNA Using a Bisulfite-Catalyzed Transamination Reaction," Nucleic Acids Research, 12(2), 989-1002 (1984).
Urdea et al., "A Comparison of Non-Radioisotope Hybridization Assay Methods Using Fluorescent, Chemiluminescent and Enzyme Labeled Synthetic Oligodeoxyribonucleotide Probes," Nucleic Acids Research, 16(12), 4937-4956 (1988).
Cook et al., "Synthetic and Hybridization of a Series of Biotinylated Oligonucleotides," Nucleic Acids Research, 16(9), 4077-4095 (1988).
Telser et al., "Syntheses and Characterization of DNA Oligomers and Duplexes Containing Covalently Attached Molecular Labels: Comparison of Biotin, Fluorescein, and Pyrene Labels by Thermodynamic and Optical Spectroscopic Measurements," J. Am. Chem. Soc., 111(18), 6966-6976 (1989).
Jablonski et al., "Preparation of Oligodeoxynucleotides--Alkaline Phosphatase Conjugates and Their Use as Hybridization Probes," Nucleic Acids Research, 14(15), 6115-6128 (1986).
Haralambidis et al., "Preparation of Base-Modified Nucleosides Suitable for Non-Radioactive Label Attachment and Their Incorporation into Synthetic Oligodeoxyribonucleotides," Nucleic Acids Research, 15(12), 4857-4876 (1987).
Gibson et al., "Synthesis and Application of Derivatizable Oligonucleotides," Nucleic Acids Research, 15(16), 6455-6467 (1987).
Zischler et al., "Non-Radioactive Oligonucleotide Fingerprinting in the Gel," Nucleic Acids Research, 17(11), 4411 (1989).
Sproat et al., "Highly Efficient Chemical Synthesis of 2'-O-Methyloligoribonucleotides and Tetrabiotinylated Derivatives: Novel Probes That are Resistant to Degradation by RNA or DNA Specific Nucleases," Nucleic Acids Research, 17(9), 3373-3386 (1989).
Allen et al., "Fluorescent Oligonucleotides and Deoxynucleotide Triphosphates: Preparation and Their Interaction with the Large (Klenow) Fragment of Escherichia coli DNA Polymerase I," Biochemistry, 28, 4601-4607 (1989).
Meyer et al., "Efficient, Specific Cross-Linking and Cleavage of DNA by Stable, Synthetic Complementary Oligodeoxynucleotides," J. Am. Chem. Soc., 111, 8517-8519 (1989).
Telser et al., "DNA Oligomers and Duplexes Containing a Covalently Attached Derivative of Tris (2,2'-bipyridine)ruthenium(II):Synthesis and Characterizations by Thermodynamic and Optical Spectroscopic Measurements," J. Am. Chem. Soc., 111, 7226-7332 (1989).
Horn et al., "Forks and Combs and DNA: The Synthesis of Branched Oligodeoxyribonucleotides," Nucleic Acids Research, 17(17), 6959-6967 (1989).
Telser et al., "DNA Duplexes Covalently Labeled at Two Sites: Synthesis and Characterization by Steady-State and Time-Resolved Optical Spectroscopies," J. Am. Chem. Soc., 111, 7221-7226 (1989.).
Burma et al., "Use of Radioactive Isotopes in the Study of Nucleic Acids," Quart. J. Surg. Sci., 5(2-3), 114-126 (1969); Chem. Abstr., 77, p. 218, Abstr. No. 7211 6e (1972); only Abstract supplied.
Altenburg et al., "Iodine 125 in Molecular Hybridization Experiments," Methods in Cell Biol., 10, 325-342 (1975); Chem. Abstr., 84, p. 185, Abstr. No. 27565j (1976); only Abstract supplied.
Sugiura et al., "Methods for Enzymatic Labeling of Nucleic Acids and Polynucleotides," Seikagaku Jikken Koza, 6(Toresa Jikkenho Pt. 2), 515-525 (1977); Chem. Abstr., 91, p. 219, Abstr. No. 188423w (1979); only Abstract supplied.
Takemura et al., "Methods for Labeling of Nucleic Acids and Polynucleotides," Seikagaku Jikken Koza, 6(Toresa Jikkenho Pt. 2), 501-513 (1977); Chem. Abstr., p. 269, Abstr. No. 206523k (1979); only Abstract supplied.
Reines et al., "A New Method for Attachment of Fluorescent Probes to tRNA," RNA Protein Synthesis, Moldave et al. (eds.), Academic Press, New York, 1981, pp. 158-168; Chem. Abstr., 97 p. 383, Abstr. No. 88013t (1982); only Abstract supplied.
Langer-Safer et al., "Immunological Method for Mapping Genes on Drosophila Polytene Chromosomes," Proc. Nat. Acad. Sci. USA, 79(14), 4381-4385 (1982); Chem. Abstr., 97, p. 383, Abstr. No. 88013t (1982); only Abstract supplied.

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