Single-stranded circular oligonucleotides useful for drug delive

Details Single-stranded circular oligonucleotides useful for drug delive Single-stranded circular oligonucleotides useful for drug delive

1995-06-06

1999-02-16

Horlick, Kenneth R.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

536 243, 536 245, A61K 4800

Patent

active

058721053

ABSTRACT:
The present invention provides single-stranded circular oligonucleotides each with at least one parallel binding (P) domain and/or at least one corresponding anti-parallel binding (AP) domain separated from each other by loop domains. When more than one P or AP domain is included in a circular oligonucleotide of the present invention, the additional P or AP domains can constitute loop domains for a pair of corresponding P and AP domains, and vice versa. The present invention further provides single-stranded circular oligonucleotides with at least one Hoogsten antiparallel (HAP) domain. Each P, AP and HAP domain has sufficient complementarity to bind to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the HAP or AP domain binds in an anti-parallel manner to the target. Moreover, the present single-stranded circular oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of making and using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.

REFERENCES:
patent: 4766062 (1988-08-01), Diamond et al.
patent: 4777129 (1988-10-01), Dattagupta et al.
patent: 5176996 (1993-01-01), Hogan et al.
patent: 5473060 (1995-12-01), Grayznov et al.
Prakash, G. and Kool, E.T. (1991). "Molecular Recognition by Circular Oligonucletides. Strong Binding o stranded DNA and RNA,"J. Chem. Soc., Chem. Commun., pp. 1161-1163.
Gura, Science 270, 575-577 (1995).
Prakash et al., J. Am. Chem. Soc. 114, 3523-3527 (1992).
Kool, J. Am. Chem. Soc. 113, 6265-6266 (1991).
Prakash et al., J. Chem. Soc., Chem. Commun. , 1161-1163 (1991).
Agrawal, et al (1988) "Oligodeoxynucleoside Phosphoramidates and Phosphorothioates as Inhibitors of Human Immunodeficiency Virus, " Proc. Natl. Acad. Sci. USA 85 : 7079-7083.
Baumann, et al (1988), "Interaction of DNA Hairpin Loops and a Complementary Strand By a Triplet of Base Pairs," Biochem. Biophys. Res. Commun. 157 :986-991.
Cooney, et al. (1988), "Site-Specific Oligonculeotide Binding Represses Transcription of the Human c-myc Gene in Vitro," Science 241 :456-459.
Durand et al (1992), "Triple-Helix Formation by an Oligonucleotide Containing One (dA).sub.12 and Two (dT).sub.12 Sequences Bridged by Two Hexaethylene Glycol Chains,"Biochemistry 31 :9197-9204.
Erie, et al (1987), "A Dumbell-Shaped, Double-Hairpin Structure of DNA: A Thermodynamic Investigation,"Biochemistry 26 :7150-7159.
Erie, et al (1989), "Melting Behavior of a Covalently Closed, Single-Stranded, Circular DNA," Biochemistry 28 :268-273.
Giovannangeli, et al (1991), "Single-Stranded DNA as a Target for Triple-Helix Formation," J. Am. Chem. Soc. 113 :7775-7777.
Goodchild, et al (1988), "Inhibition of Human Immunodeficiency Virus Replication by Antisense Oligodeoxynucleotides," Proc. Natl. Acad. Sci. USA 85 :5507-5511.
Griffin et al (1989), "Recognition of Thymine-Adenine Base Pairs by Guanine In a Pyrimidine Triple Helix Motif," Science 245 :967-971.
Lee, et al (1984), "Poly(pyrimidine)-Poly(purine) Synthetic DNA's Containing 5-methylcytosine Form Stable Triplexes at Neutral pH," Nucleic Acids Research 12 :6603-6614.
Luebke, et al (1989), "Nonenzymatic Ligation of Oligodeoxyribonucleotides on a Duplex DNA Template by Triple-Helix Formation," J. Am. Chem. Soc. 111 :8733-8735.
Maher, III et al (1991), "Oligonucleotide-Directed DNA Triple-Helix Formation: An Approach to Artificial Repressors?" Antisense Research and Development 1 :277-281.
Mendel, et al (1987), "Hoogsteen Base Pairs Proximal and Distal to Echinomycin Binding Sites on DNA," Proc. Natl. Acad. Sci. USA 84 :910-914.
Ono, et al (1991), "DNA Triplex Formation of Oligonucleotide Analogues Consisting of Linker Groups and Octamer Segments that Have Opposite Sugar-Phosphate Backbone Polarities," Biochemistry 30 :9914-9921.
Povsic, et al (1989), "Triple Helix Formation by Oligonucleotides on DNA Extended to the Physiological ph Range," J. Am. Chem. Soc. 111 :3059-3061.
Riordan, et al (1991), "Oligonucleotide-Based Therapeutics," Nature 350 :442-443.
Uhlmann, et al (1990), "Antisense Oligonucleotides: A New Therapeutic Principle, " Chemical Reviews 90 :543-584.
Vroom, et al (1988), "Synthesis of Cyclic Oligonucleotides by a Modified Phosphotriester Approach," Nucleic Acids Research 18 :4607-4620.
Xodo, et al (1990), "Spectroscopic and Calorimetric Investigation on the DNA Triplex Formed by d(CTCTTCTTTCTTTTCTTTCTTCTC) and d(GAGAAGAAAGA) at Acidic pH," Nucleic Acids Research 18 :3557-3564.

Affiliated with

Also associated with

Rating

Single-stranded circular oligonucleotides useful for drug delive does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Single-stranded circular oligonucleotides useful for drug delive, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single-stranded circular oligonucleotides useful for drug delive will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2062632