Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-06-27
1999-04-06
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
385 12, 385129, 385130, 356317, 356318, 356246, 422 55, 422 57, 422 8205, 422 8208, 422 8211, 4352871, 4352872, 4352887, 435808, 436164, 436165, 436172, 436518, 436527, 436531, 436805, G01N 3353, G01N 33543, G01N 33552
Patent
active
058916580
ABSTRACT:
Simplicity, sensitivity and versatility of optical sensors based on competitive immunoassays using antibody-antigen reactions are achieved by solid-state, single-step reactions which permit accurate sensitive qualitative and quantitative information to be obtained without human participation. All of the chemistry-biochemistry is an inherent part of the sensor. A direct reaction occurs when the sample (antigen) is brought in contact with the sensor. The sensitivity of the competitive immunoassay optical sensor is controlled and increased by selecting a tag for the antigen or altering the attachment of a tag to an antigen so that the binding of tagged antigen to an antibody is decreased relative to the binding of untagged antigen to the antibody. The user can vary size, molecular weight and geometric configuration of the tagged antigen. This can be accomplished by selecting the proper tag or by attaching the indicator material to the antigen through a spacer or by attaching the tag directly to the antigen and attaching a compound of proper molecular weight and size elsewhere. Pretreatment of the substrate to which the antibody-bound tagged antigen is immobilized to block the surface from unwanted interferences and the use of optical isolation increases sensitivity. If the tag cannot be attached to the antigen, it is attached to the antibody. Attaching the controlled size, molecular weight tag to either the antigen or antibody permits the analysis of species not normally measureable by competitive immunoassay, thus increasing the versatility of the method.
REFERENCES:
patent: 4895809 (1990-01-01), Schlabach et al.
patent: 5082630 (1992-01-01), Partin et al.
Coulter Stephen L.
Hewitt Geoffrey F.
Klainer Stanley M.
Chin Christopher L.
FCI--FiberChem, Inc.
Sartorio Henry P.
LandOfFree
Single-step, solid-state competitive immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Single-step, solid-state competitive immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single-step, solid-state competitive immunoassay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1370227