Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1993-05-10
1996-04-30
Jones, W. Gary
Chemistry: analytical and immunological testing
Biological cellular material tested
436 94, 436103, 436104, 536 2532, 5483351, G01N 3348
Patent
active
055124860
ABSTRACT:
Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##
REFERENCES:
patent: 4474876 (1984-10-01), Osband et al.
patent: 4774339 (1988-09-01), Haugland et al.
patent: 4830961 (1989-05-01), Petty
patent: 4868103 (1989-09-01), Stavrianopoulos et al.
patent: 5179210 (1993-01-01), Ebel
Ivanovskaya et al., "Modification of Oligo(poly)nucleotide Phosphomonoester Groups in Aqueous Solutions," Nucleosides & Nucleotides 6(5):913-934 (1987).
Larry J. Kricka, ed., "Nonisotopic DNA Probe Techniques," Academic Press, Inc.
Morrison et al., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization," Anal. Biochem. 183:231-244 (1989).
Sundberg et al., J. Am. Chem. Soc. 96(2); 381-392 (1974).
1991 Amersham Catalog, p. 244.
Chu et al., Nucleic Acids Res. 11(18), 6513-6529 (1983).
Al-Deen et al., J. Chromatography 512, 409-414 (1990).
Giese Roger W.
Wang Poguang
Horlick Kenneth R.
Jones W. Gary
Northeastern University
LandOfFree
Single step signal group-imidazole labeling of organic phosphate does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Single step signal group-imidazole labeling of organic phosphate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single step signal group-imidazole labeling of organic phosphate will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-628090