Single step signal group-imidazole labeling of organic phosphate

Chemistry: analytical and immunological testing – Biological cellular material tested

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

436 94, 436103, 436104, 536 2532, 5483351, G01N 3348

Patent

active

055124860

ABSTRACT:
Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##

REFERENCES:
patent: 4474876 (1984-10-01), Osband et al.
patent: 4774339 (1988-09-01), Haugland et al.
patent: 4830961 (1989-05-01), Petty
patent: 4868103 (1989-09-01), Stavrianopoulos et al.
patent: 5179210 (1993-01-01), Ebel
Ivanovskaya et al., "Modification of Oligo(poly)nucleotide Phosphomonoester Groups in Aqueous Solutions," Nucleosides & Nucleotides 6(5):913-934 (1987).
Larry J. Kricka, ed., "Nonisotopic DNA Probe Techniques," Academic Press, Inc.
Morrison et al., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization," Anal. Biochem. 183:231-244 (1989).
Sundberg et al., J. Am. Chem. Soc. 96(2); 381-392 (1974).
1991 Amersham Catalog, p. 244.
Chu et al., Nucleic Acids Res. 11(18), 6513-6529 (1983).
Al-Deen et al., J. Chromatography 512, 409-414 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Single step signal group-imidazole labeling of organic phosphate does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Single step signal group-imidazole labeling of organic phosphate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single step signal group-imidazole labeling of organic phosphate will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-628090

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.