Single stage avian embryo culture process

Details Single stage avian embryo culture process Single stage avian embryo culture process

1990-09-17

1993-11-02

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Miscellaneous

4352402, 800 2, 800DIG6, 119 68, C12N 500, A01K 6700

Patent

active

052583074

ABSTRACT:
A novel single stage culture system for avian embryos is disclosed. Premature eggs consisting of about 32 to about 100 cells are collected shortly after ovulation and prepared for culture. The eggs are incubated in a potassium rich bicarbonate solution and the contents of the eggs are transferred to recipient eggshells. The open end of the eggshell is closed and the embryos are turned and incubated in an oxygen enriched atmosphere for about 3-4 days. For the final incubation period the eggs are incubated in an upright position and rocked hourly.

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