Single-primer nucleic acid amplification methods

Details Single-primer nucleic acid amplification methods Single-primer nucleic acid amplification methods

2008-05-20

2008-05-20

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

11213519

ABSTRACT:
The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the “priming oligonucleotide,” a promoter oligonucleotide modified to prevent polymerase extension from its 3′-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The method of the present invention minimizes or substantially eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or substantially eliminates this problem, thus providing an enhanced level of sensitivity.

REFERENCES:
patent: 5130238 (1992-07-01), Malek et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5466586 (1995-11-01), Davey et al.
patent: 5554517 (1996-09-01), Davey et al.
patent: 5766849 (1998-06-01), McDonough et al.
patent: 5786183 (1998-07-01), Ryder et al.
patent: 5824518 (1998-10-01), Kacian et al.
patent: 5849497 (1998-12-01), Steinman
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 6031091 (2000-02-01), Arnold, Jr. et al.
patent: 6090591 (2000-07-01), Burg et al.
patent: 6130038 (2000-10-01), Becker et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6268490 (2001-07-01), Imanishi et al.
patent: 6410276 (2002-06-01), Burg et al.
patent: 6534274 (2003-03-01), Becker et al.
patent: 6582938 (2003-06-01), Su et al.
patent: 6670461 (2003-12-01), Wengel et al.
patent: 2003/0017591 (2003-01-01), Kurn
patent: 2003/0087251 (2003-05-01), Kurn
patent: 2004/0203019 (2004-10-01), Kurn
patent: 2007/0202523 (2007-08-01), Becker et al.
patent: 88/10315 (1988-12-01), None
Latorra et al. Design considerations and effects of LNA in PCR primers. Molec. Cell. Probes (2003) 17:253-259.
Ikeda et al., “Initiation of Transcription by T7 RNA Polymerase at Its Natural Promoters,” J. Biol. Chem., 1992, 267 (4):2640-2649, American Society for Biochemistry and Molecular Biology, Baltimore, MD, USA.
Majlessi et al., “Advantages of 2′-O-methyl oligoribonucleotide probes for detecting RNA targets,” Nucleic Acids Res., 1998, 26(9):2224-2229, Oxford University Press, Oxford, UK.
Petersen et al., “The conformations of locked nucleic acids (LNA),” J. Mol. Recognit., 2000, 13:44-53, John Wiley & Sons, Ltd., London, UK.
PCT Search Report, International Application No. PCT/US05/30329, Aug. 26, 2005.
PCT Written Opinion, International Application No. PCT/US05/30329, Aug. 26, 2005.

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