Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1998-10-09
2000-04-11
Font, Frank G.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
356237, 2504581, G01N 2164, G01J 330, F21V 916
Patent
active
060493801
ABSTRACT:
Single fluorescent molecules in a flowing sample stream are distinguished and identified using only a single laser excitation wavelength. A sample stream is formed containing a dilute mixture of single molecule fluorophores, wherein each one of the fluorophores is serially ordered in the sample stream. The sample stream is illuminated with s single excitation wavelength laser effective to excite each fluorophore one at a time. Fluorescence emission photons from each said fluorophore are detected. A burst size is determined for each fluorophore to identify each fluorophore. A pulsed laser may be used, where burst size and an intra-burst fluorescence decay rate for each fluorophore are determined simultaneously from the detected fluorescence emission photons. The burst size and the decay rate are correlated to identify each fluorophore.
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Goodwin Peter M.
Jett James H.
Keller Richard A.
Machara Nicholas P.
Van Orden Alan K.
Font Frank G.
Lauchman Layla
Regents of the University of California
Wilson Ray G.
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