Single molecule detection of bio-agents using the F1-ATPase...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091100, C435S091200

Reexamination Certificate

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06989235

ABSTRACT:
An exemplary system and method of employing DNA hybridization for the detection of bio-agents is disclosed as comprising inter alia a biomolecular rotary motor (150); a capture probe DNA fragment (140) effectively attached to said biomolecular motor (150); a target DNA fragment (130) suitably adapted for hybridization with said capture probe DNA (140); a signal probe DNA fragment (120) suitably adapted for hybridization with said target DNA (130); and a fluorescent bead (100) attached to said signal probe DNA (120). Disclosed features and specifications may be variously controlled, adapted or otherwise optionally modified to improve certain device fabrication parameters and/or performance metrics.

REFERENCES:
patent: 6232066 (2001-05-01), Felder et al.
patent: 6613516 (2003-09-01), Christians et al.
Noji et al., “Direct observation of the rotation of F1-ATPase,” Nature, Mar. 1997, vol. 386, pp. 299-302.
Frasch et al., “Molecular Motors: ATP Synthase,” on line publication, 2001, http://photoscience.la.asu.edu/bionano/research4.htm.

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