Single LTR lentivirus vector

Details Single LTR lentivirus vector Single LTR lentivirus vector

2007-05-22

2007-05-22

Priebe, Scott D. (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023100, C536S024100, C435S455000, C435S456000, C435S091410

Reexamination Certificate

active

10721563

ABSTRACT:
The present invention provides an isolated nucleic acid comprising a single retroviral LTR, a polypurine tract, a packaging signal, a primer binding site and a rev responsive element. Further provided is an isolated nucleic acid comprising a heterologous nucleotide sequence, a single retroviral long terminal repeat (LTR), a packaging signal, a rev responsive element, a polypurine tract, a eukaryotic promoter, a primer binding site, a bacterial origin of replication and a bacterial selection marker. In addition, the present invention provides an isolated nucleic acid comprising a 5′ retroviral LTR and a 3′ retroviral LTR, a heterologous nucleotide sequence, a packaging signal, a rev responsive element, a polypurine tract, a eukaryotic promoter, a primer binding site, a bacterial origin of replication and a bacterial selection marker cassette, wherein the bacterial origin of replication and bacterial selection marker are located between the two LTRs.

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