Single-chain hepatocyte growth factor variants

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C07K 14475, A61K 3818

Patent

active

055809634

ABSTRACT:
The invention concerns hepatocyte growth factor (HGF) variants that are resistant to proteolytic cleavage by enzymes capable of in vivo conversion of HGF into its two-chain form. The single-chain HGF variants, which preferably have an amino acid alteration at or adjacent to any of amino acid positions 493, 494, 495 and 496 of the wild-type human HGF sequence, retain their ability to bind the HGF receptor.

REFERENCES:
patent: 4330528 (1982-05-01), Ruhenstroth-Bauer et al.
patent: 4341765 (1982-07-01), Ruhenstroth-Bauer et al.
patent: 4851341 (1989-07-01), Hopp et al.
patent: 5004805 (1991-04-01), Gohda et al.
patent: 5179078 (1993-01-01), Rollins et al.
Seki et al. 1991 Gene 102:213-219.
Okigaki et al. 1992 Biochemistry 31:9555-9561.
Mark et al. 1992 J. Biol. Chem 267:26166-26171.
Asami et al., "Purification and Characterization of Hepatocyte Growth Factor from Injured Liver of Carbon Tetrachloride-Treated Rats", J. Biochem. 109:8-13 (1991).
Bottaro et al., "Hepatic Growth Factor Receptor is the Met Proto-Oncogene", Pat Appln. 07/885971 (Filed 18 Jan. 1991).
Bottaro et al., "Identification of the Hepatocyte Growth Factor Receptor as the c-met Proto-Oncogene Product", Science 251:802-804 (1991).
Chan et al., "Identification of a Competitive HGF Antagonist Encoded by an Alternative Transcript", Science 254:1382-1385 (1991).
Cooper et al., "Amplification and over expression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts", EMBO J. 5:2623 (1986).
Gak, E. et al., "Processing of Hepatocyte Growth Factor to the Heterodimeric Form is Required for Biological Activity," FEBS Ltrs 311:(1)17-21 (1992).
Giordano et al., "Tyrosine kinase receptor Indistinguishable from the c-met protein", Nature 339:155 (1989).
Gohda et al., "Purification and Partial Characterization of Hepatocyte Growth Factor from Plasma of a Patient with Fulminant Hepatic Failure", J. Clin. Invest. 81:414-419 (1988).
Han et al., "Characterization of the DNF15S2 Locus on Human Chromosome 3: Identification of a Gene Coding for Four Kringle Domains with Homology to Hepatocyte Growth Factor", Biochem. 30:9768-9780 (1991).
Igawa et al., "Hepatocyte Growth Factor is a Potent Mitogen for Cultured Rabbit Renal Tubular Epithelial Cells", Biochem. Biophys. Res. Commun. 174:831-838 (1991).
Lindroos et al., "Hepatocyte Growth Factor (Hepatopoietin A) Rapidly Increases in Plasma before DNA Synthesis and Liver Regeneration Stimulated by Partial Hepatectomy and Carbon Tetrachloride Administration", Hepatol 13:734-750 (1991).
Lokker et al., "Mutational Analysis and Molecular Modeling of the NK1 Domain of Hepatocyte Growth Factor: Identification of Residues that are Essential for Receptor Binding", Dept of .sup.1 Cell Genetics and .sup.2 Protein Engineering, Genentech pp. 1-35 (1993).
Lokker et al., "Structure-Function Analysis of Hepatocyte Growth Factor: Identification of Variants the lack Mitogenic Activity Yet Retain High Affinity Receptor Binding," EMBO J. 11(7):2503-2510 (1992).
Matsumoto et al., "Hepatocyte Growth Factor is a Potent Stimulator of Human Melanocyte DNA Synthesis and Growth", Biochem. Biophys. Res. Commun. 176:45-51 (1991).
Michalopoulos et al., "Control of Hepatocyte Replication by Two Serum Factors", Cancer Res. 44:4414-4429 (1984).
Miyazawa et al., "An alternatively processed mRNA generated from human hepatocyte growth factor gene", Eur. J. Biochem. 197:15-22 (1991).
Miyazawa et al., "Molecular Cloning and Sequence Analysis of cDNA for Human Hepatocyte Growth Factor", Biochem. Biophys. Comm. 163:967-973 (1989).
Montesano et al., "Identification of a Fibroblast-Derived Epithelial Morphogen as Hepatocyte Growth Factor", Cell 67:901-908 (1991).
Naka et al., "Activation of Hepatocyte Growth Factor by Proteolytic Conversion of a Single Chain Form to a Heterodimer" J. Biol. Chem. 267(28):20114-20119 (1992).
Nakamura et al., "Partial Purification and Characterization of Hepatocyte Growth Factor from Serum of Hepatectomized Rats", Biochem. Biophys. Res. Comm. 122:1450-1459 (1984).
Nakamura et al., "Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures", Proc. Natl. Acad. Sci. USA 83:6489-6493 (1986).
Nakamura et al., "Purification and subunit structure of hepatocyte growth factor from rat platelets", FEBS Letters 224:311-316 (1987).
Nakamura et al., "Molecular cloning and expression of human hepatocyte growth factor", Nature 342:440-443 (1989).
Naldini et al., "Hepatocyte growth factor (HGF) stimulates the tyrosine kinase activity of the receptor encoded by the protooncogene C-MET", EMBO J. 10:501-504 (1991).
Naldini et al., "Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor", Oxford Univ. Press 6:2867-2878 (1991).
Okajima et al., "Primary structure of rat hepatocyte growth factor and induction of its mRNA during liver regeneration following hepatic injury", Eur. J. Biochem. 193:375-381 (1990).
Park et al., "Sequence of MET protooncogene cDNA has features characteristic of the tyrosine kinase family of growth-factor receptors", Proc. Natl. Acad. Sci. USA 84:6379-6383 (1987).
Rubin et al., "A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor", Proc. Natl. Acad. Sci. USA 88:415-419 (1991).
Russel et al., "Partial Characterization of a Hepatocyte Growth Factor From Rat Platelets", J. Cell. Physiol. 119:183-192 (1984).
Seki et al., "Isolation and Expression of cDNA for Different Forms of Hepatocyte Growth Factor from Human Leukocyte", Biochem. and Biophys. Res. Commun. 172:321-327 (1990).
Shima et al., "Tumor Cytotoxic Factor/Hepatocyte Growth Factor from Human Fibroblasts: Cloning of its cDNA, Purification and Characterization of Recombinant Protein", Biochem. Biophys. Res. Comm., 180(2):1151-1158 (1991).
Stoker et al., "Scatter factor is a fibroblast-derived modulator of epithelial cell mobility" Nature 327:239-242 (1987).
Tashiro et al., "Deduced primary structure of rat hepatocyte growth factor and expression of the mRNA in rat tissues" Proc. Natl. Acad. Sci. USA 87:3200-3204 (1990).
Weidner et al., "Scatter Factor: Molecular Characteristics and Effect on the Invasiveness of Epithelial Cells", J. Cell Biol. 111:2097-2108 (1990).
Wiman, B., "Primary Structure of the B-Chain of Human Plasmin", Eur. J. Biochem. 76:129-137 (1977).
Chang et al., "Cloning and expression of a .gamma.-interferon-inducible gene in monocytes: a new member of a cytokine gene family", Int'l Immuno., 1(4):388-397 (1989).
Van Damme et al., "Structural and Functional Identification of Two Human, Tumor-derived Monocyte Chemotactic Proteins (MCP-2 and MCP-3) Belonging to the Chemokine Family", J. Exp. Med., 176:59-65 (1992).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Single-chain hepatocyte growth factor variants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Single-chain hepatocyte growth factor variants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single-chain hepatocyte growth factor variants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-786458

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.