Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-02-26
1999-09-28
Spector, Lorraine
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 695, 435 698, 435 694, 4351723, 4353201, 4352523, 435360, 4353651, 435325, 536 2351, C12N 1516, C07H 2100
Patent
active
059587377
ABSTRACT:
Single-chain forms of the glycoprotein hormone quartet, at least some members of which are found in most vertebrates, are disclosed. In one embodiment of these single-chain forms, the .alpha. and .beta. subunits of the wild-type heterodimers or their variants are covalently linked, optionally through a linker moiety. A drug may further be included within the linker moiety to be targeted to receptors for these hormones. Some of the single-chain forms are agonists and others antagonists of the glycoprotein hormone activity. Another embodiment of the single-chain compounds of the invention comprises two .beta. subunits of the glycoprotein hormones, which .beta. subunits are the same or different. These "two-.beta." forms are antagonists of glycoprotein hormone activity.
REFERENCES:
patent: 4717670 (1988-01-01), Beck
patent: 5177193 (1993-01-01), Boime
patent: 5338835 (1994-08-01), Boime
patent: 5420247 (1995-05-01), Gearing et al.
patent: 5705478 (1998-01-01), Boime
patent: 5705484 (1998-01-01), Thomason
Chemical Abstracts, vol. 115, No. 21, Nov. 25, 1991, p. 521.
Chemical Abstracts, vol. 113, No. 5, Jul. 30, 1990, p. 430.
Chemical Abstracts, vol. 108, No. 5, Feb. 1, 1988, p. 163.
Chemical Abstracts, vol. 97, No. 17, Oct. 25, 1982, p. 94.
Design of a long-acting follitropin agonist by fusing the C-terminal sequence of the chorionic gonadotropin beta submit to the follitropin beta subunit, Fares, F. et al., Proc. of the Natl. Acad. of Sci of the US, vol. 89, May 1992, pp. 4304-4308.
Comparison of the biological and immunological properties of gycosylation deficient human chorionic gonadotropin variants produced by site directed mutagenesis and chemical deglycosylation, Sairam, M. R. and Jiang, L. G., Molecular and Cellular Endocrinology, vol. 85, Jun. 1992, pp. 227-235.
Biosynthesis of a biologically active single peptide chain containing the human common alpha and chorionic gonadotropin beta subunits in tandem, Sugahara T. et al, Proc. of the Natl Acad of Sci of the U.S., (Mar. 14, 1995) 92 (6) 2041-5.
Expression of biologically active fusion genes encoding the common alpha subunit and either the CGbeta or FSHbeta subunits: role of a linker sequence, Sugahara, T. et al. Molecular and cellular endocrinology, vol. 125, 1996, pp. 71-77.
P.S. LaPolt et al., Endocrinology 131(6):2514-2520, 1992.
A.J. Lapthorn et al., Nature 369:455-461, Jun. 9, 1994.
S. Cockle et al., Protein Purification:Micro to Macro, pp. 375-381. Alan R. Liss, New York, 1987.
Ben-Menahem David
Boime Irving
Spector Lorraine
Washington University
LandOfFree
Single-chain .beta. subunit dimers of the glycoprotein hormones does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Single-chain .beta. subunit dimers of the glycoprotein hormones , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single-chain .beta. subunit dimers of the glycoprotein hormones will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-702299