Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1995-06-07
2000-03-21
Allen, Marianne P.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
530350, C12N 1512, C07K 1451
Patent
active
060404310
ABSTRACT:
Disclosed are a family of single-chain polypeptide constructs designed to agonize or mimic members of the TGF-.beta. superfamily by binding to a cell surface receptor complementary to the superfamily member. The single-chain constructs of the invention called "morphons" contain in a single biologically active subunit interacting finger and heel regions which together define a tertiary protein structure complimentary to the ligand binding surface of a receptor that binds a TGF-.beta. superfamily member. Also disclosed are truncated versions of the morphon constructs. Methods are disclosed for making and using single-chain morphons that have binding affinity for predetermined receptors of the TGF-.beta. superfamily.
REFERENCES:
patent: 4686283 (1987-08-01), Nestor, Jr. et al.
patent: 4816442 (1989-03-01), McPherson et al.
patent: 4863899 (1989-09-01), Todaro
patent: 4863902 (1989-09-01), Amagase et al.
patent: 4874746 (1989-10-01), Antoniades et al.
patent: 4929442 (1990-05-01), Powell
patent: 4968590 (1990-11-01), Kuberasampath et al.
patent: 5008240 (1991-04-01), Bentz et al.
patent: 5011691 (1991-04-01), Oppermann et al.
patent: 5013649 (1991-05-01), Wang et al.
patent: 5037643 (1991-08-01), Green
patent: 5055447 (1991-10-01), Palladino et al.
patent: 5061786 (1991-10-01), Burnier et al.
patent: 5091513 (1992-02-01), Huston et al.
patent: 5104977 (1992-04-01), Sporn et al.
patent: 5108922 (1992-04-01), Wang et al.
patent: 5108989 (1992-04-01), Amento et al.
patent: 5118791 (1992-06-01), Burnier et al.
patent: 5120535 (1992-06-01), Marquardt et al.
patent: 5141905 (1992-08-01), Rosen et al.
patent: 5187076 (1993-02-01), Wozney et al.
patent: 5258498 (1993-11-01), Huston et al.
patent: 5266683 (1993-11-01), Oppermann et al.
patent: 5284756 (1994-02-01), Grinna et al.
patent: 5316921 (1994-05-01), Godowski et al.
patent: 5322933 (1994-06-01), Davies
patent: 5804416 (1998-09-01), Wolfman et al.
Amberg et al. (19 ) "SurfZAP Vector: Linking Phenotype to Genotype for Phagemid Display Libraries," Strategies in Molecular Biology 6: 2-6.
Basler et al. (1993) "Control of Cell Pattern in the Neutral Tube: Regulation of Cell Differentiation by Dorsalin-1, a Novel TGF.beta. Family Member," Cell 73: 687-702.
Braun et al. (1988) "Transforming Growth Factor .beta. mRNA Increases During Liver Regeneration: A Possible Paracrine Mechanism of Growth Regulation," Proc. Natl. Acad. Sci. USA 85: 1539-1543.
Brunner et al. (1989) "Site-directed Mutagenesis of Cysteine Residues in the Pro Region of the Transforming Growth Factor .beta.1 Precursor," Journal of Biological Chemistry 264: 13660-13664.
Celeste et al. (1990) "Identification of Transforming Growth Factor .beta. Family Members Present in Bone-inductive Protein Purified from Bovine Bone," Proc. Natl. Acad. Sci. USA 87: 9843-9847.
Daopin et al. (1992) "Crystal Structure of Transforming Growth Factor-.beta.2: An Unusual Fold for the Superfamily," Science 257: 369-373.
Ealick et al. (1991) "Three-Dimensional Structure of Recombinant Human Interferon-.gamma." Science 252: 698-702.
Griggs et al. (1992) "The N-terminus and C-terminus of IFN-.gamma. are Binding Domains for Cloned Soluble IFN-.gamma. Receptor," Journal of Immunology 149: 517-520.
Gruppuso et al. (1990) "Transforming Growth Factor Receptors in Liver Regeneration Following Partial Hepatectomy in the Rat," Cancer Research 50: 1464-1469.
Gruppuso et al. (1991) "Fetal Growth Factors as Determinants of Intrauterine Hepatic Growth," Diabetes 40: 51-55.
Kulkarni et al. (1993) "Transforming Growth Factor .beta..sub.1 Null Mutation in Mice Causes Excessive Inflammatory Response and Early Death," Proc. Natl. Acad. Sci. USA 90: 770-774.
Ibanez et al. (1993); "An Extended Surface of Binding to Trk Tyrosine Kinase Receptors in NGF and BDNF Allows the Engineering of a Multifunctional Pan-neurotrophin," EMBO Journal 12: 2281-2293.
Lee, S. (1991) "Expression of Growth/Differentiation Factor 1 in the Nervous System: Conservation of a Bicistronic Structure," Biochemistry 88: 4250-4254.
Legerski et al. (1992) "Molecular Cloning and Characterization of a Novel Rat Activin Receptor," Biochemical and Biophysical Research Communications 183: 672-679.
Lin et al. (1993) "GDNF: A Glial Cell Line-Derived Neurotrophic Factor for Midbrain Dopaminergic Neurons," Science.260: 1130-1132.
Lowman et al. (1991) "Selecting High-Affinity Binding Proteins by Monovalent Phage Display," Biochemistry 30: 10832-10838.
Lyons, et al. (1989) "Vgr-1, A Mammalian Gene Related to Xenopus Vg-1, is a Member of the Transforming Growth Factor .beta. Gene Superfamily," Proc. Natl. Acad. Sci. USA 86: 4554-4558.
Marks et al. (1992) "Molecular Evolution of Proteins on Filamentous Phage," Journal of Biological Chemistry 267: 16007-16010.
Massague, J. (1987) "The TGF-.beta. Family of Growth and Differentiation Factors," Cell 49: 437-438.
Massague, J. (1992) "Receptors for the TGF-.beta. Family," Cell 69: 1067-1070.
McCafferty et al. (1990) "Phage Antibodies: Filamentous Phage Displaying Antibody Variable Domains," Nature : 348: 552-554.
McDonald et al. (1991) "New Protein Fold Revealed by a 2.3-.ANG. Resolution Crystal Structure of Nerve Growth Factor," Nature 354: 411-414.
McDonald et al. (1993) "A Structural Superfamily of Growth Factors Containing a Cysteine Knot Motif," Cell 73: 421-424.
McPherron et al. (1993) "GDF-3 and GDF-9: Two New Members of the Transforming Growth Factor-.beta. Superfamily Containing a Novel Pattern of Cysteines," The Journal of Biological Chemistry 268: 3444-3449.
Mead et al. (1989) "Transforming Growth Factor .alpha. may be a Physiological Regulator of Liver Regeneration by Means of an Autocrine Mechanism," Proc. Natl. Acad. Sci. USA 86: 1558-1562.
Miyazono et al. (1993) "Transforming Growth Factor-.beta.: Latent Forms, Binding Proteins and Receptors," Growth Factors 8 : 11-22.
Oefner et al. (1992) "Crystal Structure of Human Platelet-derived Growth Factor BB" The EMBO Journal 11: 3921-3926.
Ozkaynak, et al. (1990) "OP-1 cDNA encodes an osteogenic protein in the TGF-.beta. Family" The EMBO Journal 9: 2085-2093.
Pepinsky et al. (1988) "Proteolytic Processing of Mullerian Inhibiting Substance Produces a Transforming Growth Factor-.beta.-like Fragment," Journal of Biological Chemistry 263: 18961-18964.
Qian et al. (1992) "Identification of a Structural Domain that Distinguishes the Actions of the Type 1 and 2 Isoforms of Transforming Growth Factor .beta. on Endothelial Cells," Proc. Natl. Acad. Sci. USA 89: 6290-6294.
Schlunegger et al. (1990) "An Unusual Feature Revealed by the Crystal Structure at 2.2.ANG. Resolution of Human Transforming Growth Factor-.beta.2," Nature 358: 430-434.
Smith et al. (1990) "Automatic Generation of Primary Sequence Patterns From Sets of Related Protein Sequences," Proc. Natl. Acad. Sci. USA 87: 118-122.
Wells et al. "Optimizing Binding and Catalysis by Phage Display," Advances in Gene Technology: Protein Engineering and Beyond pp. 105.
Wharton et al. (1991) "Drosophila 60A Gene, Another Transforming Growth Factor .beta. Family Member, is Closely Related to Human Bone Morphogenetic Proteins," Proc. Natl. Acad. Sci USA 88: 9214-9218.
Honigwachs-Sha 'Anani et al. "Restrictin-P: The First Member of a Putative Family of Novel Inhibitors", Annals New York Academy of Sciences pp. 287-297.
Keck Peter C.
Smart John E.
Allen Marianne P.
Stryker Corporation
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