Single apparatus for slab gel electrophoresis and blotting

Details Single apparatus for slab gel electrophoresis and blotting Single apparatus for slab gel electrophoresis and blotting

1989-08-22

1991-02-19

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041821, 2041828, 204301, G01N 2726, B01D 5702

Patent

active

049941666

ABSTRACT:
Electrophoretic separation in a slab gel and blotting of the resulting zone pattern onto a membrane are both performed in a single tank cell which contains separation electrodes along opposing vertical walls and blotting electrodes horizontally arranged above and below the level designated for the gel placement. The blotting electrode situated below the gel is an array of parallel wires arranged to simulate a plate electrode. The cell is operated in separatory and blotting modes, in which the separation and blotting electrodes, respectively, are separately energized. During the separatory mode, the wires in the array forming the lower blotting electrode are electrically isolated from each other to permit each to adjust to the voltage of the buffer immediately surrounding it.

REFERENCES:
patent: 4622124 (1986-11-01), Kreisher et al.
Reijenga, J. C.; Verheggen, Th. P .E. M.; Everaerts, F. M. "Fluorescence Emission and Fluorescence Quenching as Detection Methods in Isotachophoresis"Journal of Chromatography, 283 (1984), 99-111.

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