Simultaneous radioassay of folate and vitamin B12

Details Simultaneous radioassay of folate and vitamin B12 Simultaneous radioassay of folate and vitamin B12

1981-02-12

1983-12-27

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436545, 436804, G01N 3356, G01N 3360, B07D 7100

Patent

active

044231547

ABSTRACT:
A serum sample is heated at an alkaline pH to release folate and vitamin B.sub.12 from endogenous binders. A simultaneous radioassay for folate and vitamin B.sub.12 is effected by contacting the sample with binder for folate, binder for vitamin B.sub.12, folate labeled with one radioactive isotope and vitamin B.sub.12 labeled with another radioactive isotope, followed by separation of bound and free portions, and determination of the radioactivity of at least one of the portions. The amounts of folate and vitamin B.sub.12 present in the sample may be determined from standard curves.

REFERENCES:
patent: 3937799 (1976-02-01), Lewin et al.
patent: 3952091 (1976-04-01), Grunberg et al.
patent: 3972991 (1976-08-01), Caston et al.
patent: 3981863 (1976-09-01), Niswender et al.
patent: 3988431 (1976-10-01), Givas et al.
patent: 4146602 (1979-03-01), Gutcho et al.
patent: 4279859 (1981-07-01), Gutcho et al.

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