Simultaneous radioassay of folate and vitamin B.sub.12

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

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424 1, 424 12, 23230B, B65D 7100, G01N 3348

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active

042798590

ABSTRACT:
A serum sample is heated at an alkaline pH to release folate and vitamin B.sub.12 from endogenous binders. A simultaneous radioassay for folate and vitamin B.sub.12 is effected by contacting the sample with binder for folate, binder for vitamin B.sub.12, folate labeled with one radioactive isotope and vitamin B.sub.12 labeled with another radioactive isotope, followed by separation of bound and free portions, and determination of the radioactivity of at least one of the portions. The amounts of folate and vitamin B.sub.12 present in the sample may be determined from standard curves.

REFERENCES:
patent: 4146602 (1979-03-01), Gutcho et al.

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