Simultaneous quantification of PIG-A associated proteins in...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007230, C435S007240, C435S007250, C435S973000, C436S010000, C436S063000, C436S064000, C436S172000, C422S073000, C422S082050

Reexamination Certificate

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07358059

ABSTRACT:
A single measurement can quantify phosphatidyl-inositol glycan complementation class A (PIG-A) associated proteins in red cells, platelets and the major leukocyte subsets present in blood (lymphocytes, monocytes, neutrophils and eosinophils). The single measurement relies on flow cytometry to simultaneously evaluate intensity of fluorochrome emissions and light scattering properties.

REFERENCES:
patent: 6287791 (2001-09-01), Terstappen et al.
Richards et al., Application of Flow Cytometry in the Diagnosis of Paroxysmal Nocturnal Hemoglobinuria, Cytometry 42: 223-233 (2000).
Griscelli-Bennaceur et al., Aplastic Anemia and Paroxysmal Nocturnal Hemoglobinuria: Search for a pathogenic Link, Blood 85 (5): 1354-1363 (Mar. 1, 1995).

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