Simultaneous extraction of a ligand from a sample and capture by

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436518, 436530, 436531, 436804, G01N 33535, G01N 33545, G01N 33569

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active

047940760

ABSTRACT:
Methods and kits for performing a ligand/anti-ligand assay are described. The ligand/anti-ligand assay comprises: (1) simultaneously carrying out the extraction of a ligand from a sample of cells or cells and reaction of the ligand with at least two anti-ligands therefor to form a detectable reaction product, and (2) detecting the reaction product.

REFERENCES:
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Ross, P. W. et al., J. Clin. Pathol., 33, 691-693, (1980).
Kaplan, L. E., et al., J. Clin. Micro., 14 (6):678-680, 1981.
Cumming, C. G. et al., J. Med. Micro., 13:459-461, 1980.
Facklam, R. R. et al., J. Clin. Micro., 15 (6), 987-990, 1982.

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