Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1987-06-15
1989-09-26
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 7, 435810, 436510, 436518, 436531, 436800, 436805, 436808, 436809, 436811, 436825, 436826, G01N 33569, G01N 33535, G01N 33545
Patent
active
048700037
ABSTRACT:
A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or "spike" of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.
REFERENCES:
patent: 4172117 (1979-10-01), Schober
patent: 4661445 (1987-04-01), Saxinger et al.
patent: 4748110 (1988-05-01), Paul
Allman David M.
Forman Meryl A.
Hofheinz David E.
Kortright Kenneth H.
Lee Song Y.
Cass Myron C.
Coulter Corporation
Nucker Christine M.
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