Simultaneous enumeration of E. coli and total coliforms

Details Simultaneous enumeration of E. coli and total coliforms Simultaneous enumeration of E. coli and total coliforms

1994-04-29

2000-11-14

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 34, C12Q 154

Patent

active

061468401

ABSTRACT:
The general method involves contacting a bacterial colony with a .beta.-D-galactosidase substrate and a carbon source metabolizable by a plurality of coliform species but not metabolizable by E. coli, and detecting reaction product signals of the substrate and carbon source. Typically, the carbon source is a carbohydrate, preferably adonitol, esculin, salicin, amygdalin, or cellobiose. The absence of both reaction product signals indicates the presence of non-coliform bacteria; the presence of both reaction product signals indicates the presence of non-E. coli coliform bacteria; and, the presence of the .beta.-D-galactosidase substrate reaction product signal and absence of the carbon source reaction product signal indicates the presence of E. coli.
A signal may identify a reaction product itself (e.g. a colored product derived from a chromogenic form of the substrate or from a chromogenic reagent which interacts with the product, etc.), or an effect of a reaction product on or at the colony (e.g. catabolite repression, growth, localized pH change, etc.). Preferably, the .beta.-D-galactosidase substrate reaction product signal is provided directly by a .beta.-D-galactosidase reaction product and the carbon source reaction product signal is provided by carbon source reaction product-induced catabolite repression of the .beta.-D-galactosidase substrate reaction product signal at a portion of the colony.

REFERENCES:
patent: 4591554 (1986-05-01), Koumura
patent: 4925789 (1990-05-01), Edberg
patent: 5210022 (1993-05-01), Roth
"Dehydrated Culture Media and Reagents for Microbiology", DIFCO Manual, Tenth Edition, DIFCO Laboratories, Detroit, Michigan.
Atlas, "Handbook of Microbiological Media", CRC Press, Lawrence C. Parks (Ed.).
Rambach, "New Plate Medium for Facilitated Differentiation of Salmonella spp. from Proteus spp. and Other Enteric Bacteria", Applied and Environmental Microbiology, 56(1):301-303 (1990).
DIFCO Manual 10th Ed DIFCO Labs Detroit MI 1984 pp. 203-204.
Chang, G., Tactics for Combining the Coliform . . . J of Food Protection vol. 53 Oct. 1990 p. 910.
Chang, G., Tryptophaw & Gacactoside Media . . . Soc Microbiol 90(0) 1990 p. 290.
Bainbridge B., Improved Methods for the Detection . . . FEMS Micro Lett 80 (1991) pp. 319-324.
Atlas R., Handbook of Microbiological Media CRC Press FL pp. 132, 178.

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