Simultaneous dual excitation/single emission fluorescent sensing

Chemistry: analytical and immunological testing – Oxygen containing – Inorganic carbon compounds

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436163, 436172, 422 8208, G01N 2164

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active

056725159

ABSTRACT:
A simultaneous dual excitation/single emission ratiometric method using fiber optic sensors is provided for detecting or measuring a parameter of interest in a sample, including pH, concentration of gases such as CO.sub.2, and the like. The method involves simultaneously providing modulated optical light corresponding to first and second regions of an indicator species' absorption or excitation spectrum, detecting modulated emission signals from the indicator species, demodulating the emission signals, calculating the apparent quantity of analyte present in the sample from the indicator emission signals and correcting the apparent quantity of analyte present for variations resulting from external factors, by determining the ratio of the demodulated indicator emission signals. An apparatus is provided for performing the method as well.

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