Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-02-14
1993-09-07
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 79, 435810, 435972, 436501, 436518, 436523, 436536, 436538, 436540, 436819, G01N 3353, G01N 33543
Patent
active
052428044
ABSTRACT:
A simultaneous dual analyte assay for determining the fertile period of the human menstrual cycle. The assay utilizes a capture reaction component consisting of P-3-G immobilized on a microporous membrane, a blocking reaction component consisting of anti E.sub.1 -3-G antibody, a labelled reaction component consisting of gold particle labelled anti E.sub.1 -3-G antibody, and an ambifunctional reaction component consisting of a hybrid immunoreactive substance having an anti P-3-G antibody binding site and a plurality of E.sub.1 -3-G determinant binding sites. An aqueous sample containing P-3-G and E.sub.1 -3-G is contacted with the components and the assay is calibrated to provide a positive assay result only when the concentration of P-3-G in the sample is less than a predetermined concentration and the concentration of E.sub.1 -3-G in the sample is more than a preselected concentration whereby a visually inspectable quantity of an immunocomposite is produced that consists of both the P-3-G immobilized on the microporous membrane and the gold labelled anti E.sub.1 -3-G antibody.
REFERENCES:
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Nakane, P. K., et al., J. Histochem. & Cytochem., vol. 22, No. 12, pp. 1084-1091 (1974).
Dictionary of Immunology, Third Edition, W. J. Herbert, ed. Blackwell Scientific Publications, Oxford, UK, pp. 165 & 17, 1987.
Dictionary of Immunology, ed. W. J. Herbert, Blackwell Scientific Publications, Osney Mead, Oxford, UK, p. 187 (1985).
Bahar Izak
Cannon L. Edward
Cole Francis X.
Hygeia Sciences, Inc.
Kepplinger Esther L.
Scheiner Toni R.
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