Simultaneous detection of leukocyte phagocytic and killing abili

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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2504612, 356 39, 436 63, 436164, 436172, G01N 3348, G01N 3349

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045813345

ABSTRACT:
Methods for the determination of phagocytic and killing ability of selected leukocyte subclasses based upon the use of three part differentials obtained by light scatter characteristics and gated fluorescence. Particles having multiple fluorescent labels associated therewith, one of which is sensitive to reactive oxygen, incubated with the leukocytes. Detection of the fluorescent labels permits identification of cells having phagocytic ability, as determined by presence of the non-sensitive label, and also the identification of those with killing ability as determined by the decrease in fluorescence from the sensitive label.

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Hultborn, R., et al., "Studies on Leucocyte Function by Measuring Respiration and Nitroblue Tetrazolium Reduction by Simplified Methods", Scand. J. Clin. Lab. Invest., 23, 297 (1973).

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