Simultaneous assay for glucose and urea

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving urea or urease

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435 14, 435 25, 435 26, 435 18, 435 4, 435 15, 436 95, 436108, C12Q 158, C12Q 154, C12Q 136, C12Q 132

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050377388

ABSTRACT:
A method for the simultaneous determination of glucose and urea in a specimen with a single reagent system. A reagent system containing a reactant for each of glucose and urea to be determined is added to a specimen. The reagent system is reacted with the specimen such that each of glucose and urea react with their respective reactant simultaneously. Each reactant is selected such that it is capable of giving an absorbance band for glucose and urea which permits their simultaneous determination. The change in absorbance or fluorescence of the resulting reaction mixture is monitored at a plurality of wavelengths which are characteristic for each of glucose and urea.

REFERENCES:
patent: 3964870 (1976-06-01), Tiedemann et al.
patent: 4425427 (1984-01-01), Luderer
Fu, P. C. et al., Adv. Autom. Anal., Technicon Int. Congr. (1973), vol. 1, pp. 171-178.
Fu, P. C. et al., Chemical Abstracts, vol. 82, No. 21, Abstract No. 135224 q (1975).
Kerscher, L. et al., "Urea", in Methods of Enzymatic Analysis, vol. VIII, 3 ed., (Bergmeyer, ed.) Weinheim, FRG, pp. 444-448 (1984).
Mollering, H. et al., "Visualization of NAD(P)-Dependent Reactions" in Methods of Enzymatic Analysis, vol. I, 2 ed., (Bergmeyer, ed.), FRG, pp. 136-144 (1974).
Vormbrock, R "UV-Method with Glucose Dehydrogenase", in Methods of Enzymatic Analysis, vol. VI, (Bergmeyer, ed.), 3 ed., FRG, pp. 172-178 (1984).

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