Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-11-21
1999-11-30
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436512, 436513, 436529, 436800, 5303911, 5303913, 5303915, G01N 33548
Patent
active
059940891
ABSTRACT:
A method for a single-measurement quantification of multiple populations of white blood cells (WBC) is based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye. The phycobiliproteins upon laser excitation produce a different detectable fluorescence intensity for each cell population. Use of such pairs of conjugates enable two populations of cells with similar receptor densities to be distinguished with the use of a single color marker. Further use of additional ligands to other cell surface receptors and additional phycobiliprotein or tandem dye, or other markers in the same manner, enables the simultaneous quantification of up to 45 different cell populations with one laser line and four fluorescent colors.
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U.S. Application No. 08/857,941, filed May 16, 1997.
Burshteyn Alexander
Mylvaganam Ravindra
Siiman Olavi
Wilkinson Julie
Bak Mary E.
Chin Christopher L.
Coulter International Corp.
Kurz Warren W.
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