Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2005-12-22
2009-08-04
Weber, Jon P (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S325000, C435S383000
Reexamination Certificate
active
07569380
ABSTRACT:
In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
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Bennett George N.
Dittrich Cheryl Renee
San Ka-Yiu
Sanchez Ailen
Baker & McKenzie LLP
Gebreyesus Kagnew H
Rice University
Weber Jon P
LandOfFree
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