Liquid purification or separation – With means to add treating material – Chromatography
Reexamination Certificate
2005-07-19
2005-07-19
Therkorn, Ernest G. (Department: 1723)
Liquid purification or separation
With means to add treating material
Chromatography
C210S502100, C210S635000, C210S656000, C502S404000, C502S439000
Reexamination Certificate
active
06919021
ABSTRACT:
A method and compound for the purification of proteins including the attachment of a non-peptidic, small compound which simulates the affinity of Protein A for immumoglobulins to a support matrix. Once attached on the support matrix, the resulting monochloro-triazine derivative is reacted with an excess of an amino compound at a higher temperature to achieve high levels of substitution. The resulting support matrix with ligand is useful in the affinity separations of antibodies. Further, a mercapto heterocyclic system ligand may be attached to the super matrix and useful in affinity separations of antibodies.
REFERENCES:
patent: 5240602 (1993-08-01), Hammen
patent: 5942463 (1999-08-01), Oscarsson et al.
patent: 6315900 (2001-11-01), Stipanovic et al.
patent: WO 91/03257 (1991-03-01), None
patent: WO 97/10887 (1997-03-01), None
Schwarz, Alexander,Five-membered Mercaptoheterocyclic Ligands for Thiophilic Adsorption Chromatography, J. Molecular Recognition 9: 672-674, 1996.
Li, Rongxiu, Victor Dowd, David J. Stewart, Stephen J. Burton, and Christopher R. Lowe,Design, synthesis, and application of a Protein A mimetic, Nature Biotechnology 16: 190-195, 1998.
Fassina, Giorgio, Antonio Verdoliva, Maria Risaria Odierna, Menotti Ruvo and Giovanni Cassini,Protein A mimetic Peptide Ligand for Affinity Purification of Antiobiotics, Journal of Molecular Recognition 9: 564-569, 1996.
Griffin Martin
Scarpa Ioannis
Stipanovic Bozidar
Accurate Polymers, Ltd.
Fitch Even Tabin & Flannery
Therkorn Ernest G.
LandOfFree
Simulated activity of Protein A displayed by ligands... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Simulated activity of Protein A displayed by ligands..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Simulated activity of Protein A displayed by ligands... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3409125