Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-05-19
1998-10-13
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 30, 435 34, 435174, 435176, 435177, 435180, 436518, 436525, G01N 3353
Patent
active
058210660
ABSTRACT:
A rapid method for the detection, identification and enumeration of specific respiring microorganisms. The method includes steps of a) passing a microbial sample through a collecting device to capture the cells; b) adding to the collecting device a fluorochrome dye specific for the detection of respiring cells and allowing the dye to incubate; c) treating the collecting device with a reactive fluorescent antibody which reacts with a target microorganism of interest present in said microbial sample; d) mounting the collecting device for examination by fluorescence microscopy in which a suitable light system is used to excite the fluorochrome dye and fluorescent antibody to fluoresce; and e) quantifying the respiring cells. Alternative embodiments include the use of immunomagnetic beads and other means of cell capture, and employing fluorescent oligonucleotide probes rather than fluorescent antibodies.
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McFeters Gordon A.
Pyle Barry H.
Duffy Patricia A.
Hutzell Paula K.
The Research & Development Institute, Inc.
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