Simple, rapid and reliable method for detecting thalassemia

Details Simple, rapid and reliable method for detecting thalassemia Simple, rapid and reliable method for detecting thalassemia

1992-10-23

1994-01-25

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168, C12P 1934

Patent

active

052815196

ABSTRACT:
A simple, rapid and reliable method for diagnosis of thalassemia is described. The method comprises amplification of the cDNA by polymerase chain reaction and determining the ratio between .alpha. and .beta. hemoglobin chain mRNAs.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Kazazian, Jr. et al., PNAS (USA) 72(2):567-571 (Feb. 1975).
Maniatis et al. Molecular Cloning & Laboratory Manual, Cold Spring Harbor Lab., NY, 1982, pp. 188-189.
Bethesda Research Labs Catalog & Reference Guide, 1988, p. 139.
Saiki et al., The New Engl. J. Med. 319(9):537-541 (Sep. 1, 1988).

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