Simple PCR technique for detecting and differentiating bacterial

Details Simple PCR technique for detecting and differentiating bacterial Simple PCR technique for detecting and differentiating bacterial

1996-10-28

1999-09-28

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

059586869

ABSTRACT:
A simple polymerase chain reaction procedure is described for the detection and differentiation of Shigella from other pathogenic Escherichia coli isolates, such as EIEC and EPEC. Serotype specific primers derived from the rfc genes of different Shigella strains are used to identify the most prominents Shigella serotypes, such as S. sonnei, S. flexneria 1 through 5, and S dysenteriae 1. More than 95% of Shigellosis cases reported could be identified by the serotype specific primers described.

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