Simple method for quantitative measuring the adhesion of...

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

Reexamination Certificate

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C436S524000, C436S527000, C436S534000, C436S010000, C436S017000, C436S018000, C436S063000, C436S069000, C436S165000, C435S002000, C435S286700, C422S073000

Reexamination Certificate

active

07485472

ABSTRACT:
The invention relates to measuring of the adhesion of platelets ex vivo. In this method, monodisperse polymer particles are added to blood, and the number of platelets in the blood are measured before and after shaking of the sample. The level of adhesion of the platelets is determined by comparing the number of platelets prior to shaking with the number of platelets after shaking. Methods of establishing an adhesion index and diagnosis of a patient are also provided.

REFERENCES:
patent: 5576185 (1996-11-01), Coulter et al.
patent: 5773228 (1998-06-01), Reed et al.
patent: WO97/18474 (1997-05-01), None
Bonnefoy et al., Efficiency of Platelet Adhesion to Fibrinogen Depends on both Cell Activation and Flow, Biophysical Journal, 78: 2834-2843 (Jun. 2000).
Bombeli et al., Adhesion of Activated Platelets to Endothelial Cells: Evidence for a GPIIbIIIa-dependent Bridging Mechanism and Novel Roles for Endothelial Intercellular Adhesion Molecule I (ICAM-1), αv β3 Integrin, and GPIbα, J. Exp. Med. 187 (3): 329-339 (Feb. 2, 1998).

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