Simple and rapid method for detection of virulent Yersinia enter

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 29, 435 6, 435 39, 435320, 435822, 436 63, 436164, 424 3, 424 71, C12Q 100, C12Q 102, G01N 3348, G01N 130

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049524976

ABSTRACT:
A crystal-violet dye binding technique useful for the detection and differentiation of virulent plasmid-bearing strains of Yersinia enterocolitica. Virulent plasmid-bearing strains of the bacteria bind the crystal violet dye to form dark violet colonies while avirulent plasmidless strains fail to bind the dye and remain white in color. The method is simple, rapid, economical and highly reliable.

REFERENCES:
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J. J. Jagow et al., "Enumeration by DNA Colony Hybridization of Virulent Yersinia enterocolitica Colonies in Artificially Contaminated Food," Appl. Environ. Microbiol. 51: 441-443 (1986).
D. A. Sack et al., "Test for Enterotoxigenic Escherichia coli Using Y1 Adrenal Cells in Miniculture," Infect. Immun. 11: 334-336 (1975).
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