Simmondsin concentrate from jojoba

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex

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426430, 426478, 426484, A61K 3578

Patent

active

060078235

ABSTRACT:
A process for the isolation of simmondsins from jojoba meal is disclosed. Simmondsins are first extracted from defatted jojoba meal with water. After separation from the meal, this water extract is subsequently dried, leaving the simmondsins in the resulting solid. The simmondsins may then be preferentially extracted from the dried water extract by contact with a first ethanolic solvent, forming a first solvent fraction containing simmondsins therein. The first solvent fraction, which is a substantially protein-free concentrate of simmondsins, may be separated from the solid phase, and the ethanolic solvent may then be readily removed, for example, by drying. Residual simmondsins remaining in the meal following extraction with the first ethanolic solvent may also be recovered by a second extraction with ethanolic solvent. Optionally, simmondsin and its analogs, simmondsin ferulate, demethylsimmondsin, and didemethyl simmondsin, may be separated in substantially pure form from the extracts by reverse-phase HPLC.

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Hassaneen, Nagwa Zaki Sadek, "Extraction of Oilssed Model Systems with Alcohols", Dissertation Grad. College of Texas A&M Univ., Aug. 1985.
Lanzani, A., "A Wet Process Technology Applied to Jojoba Seed to Obtain Oil and Detoxified Protein Meal", JAOCS 60 (10): 722-774, Oct. 1991.
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Van Boven, M. et al., "Isolation, Purification and Stereochemistry of Simmondsin", Jour. of Agric & Foood Chem. 41 (10) : 1605-1607, Oct. 1993.
Van Boven, M. et al. "Isolation and Structure Elucidation of the Major Simmondsin Analogues in Jojoba Meal by Two-Dmensional NMR Spectroscopy", Jour. of Agric. & Food Chem. 42 (12): 2684-2687, Dec. 1994.
Van Boven, M. et al., "New Simmondsin 2'-Ferulate from Jojoba Meal", Jour. of Agric. & Food Chem. 43 (10) : 1193-1197, May 1995.
Van Boven, M. et al., "Determination of Simmondsins and Simmondsin Ferulates in Jojoba Meal and Feed by High-Performance Liquid Chromotography", Jour. of agric. & Food Chem. 44 (8) : 2239-2243, Aug. 1996.
Verbiscar, A. et al., "Detoxification of Jojoba Meal", Jour. of Agric. & food Chem. 28 (3): 571-578, 1980.
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