Silver stains for protein in gels

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

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436169, 436174, 436905, G01N 3368

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active

044057202

ABSTRACT:
A silver stain method for polypeptides in gels comprising the sequential steps of photo-reversing the polypeptide-gel by treatment with an oxidizing agent, forming a latent stain image by treating the polypeptide-gel with a photosensitive salt, and developing the stain image by treating the polypeptide-gel with a reducing agent.

REFERENCES:
patent: 3873433 (1975-03-01), Seidel et al.
patent: 4167467 (1979-09-01), Golias
C. R. Merril et al., Anal. Biochem., 110(1), 201-207, (Jan. 1, 1981).
Kerenyi, et al., "A Highly Sensitive Method for Demonstrating Proteins in Electrophoretic, Immuno-electrophoretic and Immuno-diffusion Preparations", Clin. Chim. Acta, 38, 465-467, (1972).
Kerenyi, et al., "Uber Probleme der Quantitiven Auswertung der mit Physikalischer Entwicklung Versilberten Agarelektrophoretogramme", Clin. Chim. Acta, 47, 425-436, (1973).
Veerheecke, "Agargel Electrophoresis of Unconcentrated Cerebrospinal Fluid", J. Neurol., 209, 59-63, (1975).
Karcher, et al., "Cerebrospinal Fluid Proteins Electrophoresis without Prior Concentration", Acta nuerol. Belg., 79, 335-337, (1979).
Switzer, et al., "A Highly Sensitive Silver Stain for Detecting Proteins and Peptides in Polyacrylamide Gels", Anal. Biochem, 98, 231-237, (1979).
Merril, et al., "Trace Polypeptides in Cellular Extracts and Human Body Fluids Detected by Two-Dimensional Electrophoresis and a Highly Sensitive Silver Stain", Proc. Natl. Acad. Sci. U.S.A., 76, 4335-4339, (1979).
Oakley, et al., "A Simplified Ultrasensitive Silver Stain for Detecting Proteins in Polyacrylamide Gels", Anal. Biochem. 105, 361-363, (1980).

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